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Determination of natural tocopherols and added vitamin e in dietetic seed oils

, : Determination of natural tocopherols and added vitamin e in dietetic seed oils. Rivista Italiana delle Sostanze Grasse 66(1): 3-10

A HPLC method has been developed for the determination of the naturally occurring tocopherols and of the alpha-tocopheryl acetate added in dietetic seed oils. The chromatographic system utilizes a 10 .mu.m RP-18 column, a methanol/water (96:4) mobile phase and a variable wavelength UV detector set at 288 nm. Samples are dissolved in ethyl acetate and filtered (0.45 .mu.m) prior to their injection in the chromatograph, without any further treatment. This method allows the determination of tocopherols both in refined and crude seed oils.


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