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Dopamine content and metabolism in mesencephalic and diencephalic cell cultures: sex differences and effects of sex steroids


, : Dopamine content and metabolism in mesencephalic and diencephalic cell cultures: sex differences and effects of sex steroids. Journal of Neuroscience 11(5): 1325-1333

Sexual differentiation of dopaminergic neurons was studied in gender-specific cultures. Dissociated cell cultures were prepared from di- or mesencephalon of gestational day 14 rat embryos and raised in the absence or presence of 17.beta.-estradiol or testosterone for up to 13 days in vitro (DIV). Developmental profiles of levels of dopamine (DA) and metabolites as well as capacity for vesicular storage of the transmitter were determined by HPLC. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were counted. Higher levels of DA were measured in female than in male cultures of both brain regions. In mesencephalic cultures, the differences in DA levels were fully accounted for by sex differences in numbers of TH-IR cells, whereas no sex differences in cell numbers were found in diencephalic cultures. Dihydroxyphenylacetic acid (DOPAC) levels and vesicular storage capacity matured faster in mesencephalic than in diencephalic cultures, but no sex differences were observed. Homovanillic acid (HVA) could not be detected except in 13-DIV mesencephalic cultures. Hormonal treatment did not erase sexual differentiation of dopaminergic neurons. Irrespective of the gender, however, both steroids decreased DA and DOPAC contents in diencephalic cultures but not in mesencephalic cultures. It is proposed that sexual differentiation of dopaminergic systems proceeds in a region-specific fashion amd that neurogenesis and development of various parameters of dopaminergic activity may be differentially affected. Sexual differentiation of dopaminergic neurons may be initiated independently of the action of gonadal steroid hormones and may subsequently be modified by differences in hormonal environment.

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PMID: 1674002


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Glitsch, H.G.; Reuter, H.; Scholz, H., 1970: The effect of the internal sodium concentration on calcium fluxes in isolated guinea-pig auricles. 1. Calcium efflux from guinea-pig auricles followed saturation kinetics when [Ca](o) and [Na](o) were changed while the ratio [Ca](o)/[Na](o) (2) was kept constant. The Michaelis constant, K(m) (Ca+Na) = 40 mM, suggests that a hypothetical carrier...

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Woodruff W.W.IIi; Hoeck B.E.; Chitwood W.R.Jr; Lyerly H.K.; Sabiston D.C.Jr; Chen J.T.T., 1984: Radiographic findings in pulmonary artery hypertension with chronic pulmonary embolism. Radiology 153(SPEC ED): 80

Schneider, G.; Seidel, R.; Altmeyer, K.; Remberger, K.; Pistorius, G.; Kramann, B.; Uder, M., 2001: Lymphangioma of the pancreas and the duodenal wall: MR imaging findings. Pancreatic lymphangiomas are rare benign tumours with a histogenesis not yet completely understood. Predominantly the cystic aspect of this lesion can complicate the differentiation from other neoplastic and non-neoplastic cystic tumours of the pa...

Tanemoto, K.; Takao, N.; Kageshita, S.; Adachi, K.; Nishida, K., 1975: Normalization of electrocardiographic manifestations in myocardial infarct. Japanese Circulation Journal 39(7): 841-841

Kim, C.H.; Kwon, O Seob; Lee, J.A., 2003: A study on the lysis of a bluegreen alga Anabaena cylindrica by a bacterium. A Gram (-), rod-shaped bacterium in size of 1.6-2.8 X 0.4 mum was isolated from a eutrophic reservoir, which exhibited growth-inhibiting effect against a bluegreen alga (Anabaena cylindrica). This isolate showed positive reactions for catalase and...

D.M.y J.; D.R.dder W.; Vanhoutte P.M., 1980: Different potencies of alpha adrenergic antagonists in canine arteries and veins. Blood Vessels 17(3): 148

Pan-Yu-Hong; Huang-Xin-Hong; Zhuo-De-Xiang, 2001: Analysis of Enterococcus spp. resistance to antibiotics. Eighty eight clinical isolates of Enterococcus spp. in our hospital were collected and their resistance to antibiotics as well as the relationship between the pathogens and the incidence of infectious diseases were also investigated. The identific...