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Effects of temperature carrier composition and sample load in asymmetrical flow field flow fractionation

, : Effects of temperature carrier composition and sample load in asymmetrical flow field flow fractionation. Journal of Chromatography 548(1-2): 393-406

The use of asymmetrical flow field-flow fractionation for accurate sample characterization requires good control of side effects which can adversely influence sample retention. Some of these factors are identified and means to avoid or minimize them are given. The behaviour of a model protein and virus was investigated. Bad reproducibility of the retention times and peak deformations were traced to incomplete temperature control and adsorption to the accumulation wall membrane. Good thermostatic control gave constant retention times because it eliminated temperature variations caused by friction heat produced in the carrier stream. Rinsing of the channel removed adsorbed species. The mechanism involved in the adsorption is unknown but the pH of the carrier liquid appears to have an influence. Exceeding the sample load limit leads to a complex behaviour of peak deformations. This behaviour as well as the load limit is governed by the choice of buffer and ionic strength of the carrier liquid.


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