+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

ELISA analysis of BACTEC bottles for the earlier diagnosis of tuberculosis

, : ELISA analysis of BACTEC bottles for the earlier diagnosis of tuberculosis. American Review of Respiratory Disease 140(3): 668-671

Enzyme-linked immunosorbent assays (ELISA) have enabled earlier identification of Mycobacterium tuberculosis (TB) in clinical settings by utilizing both TB antibody and antigen detection. We studied the sensitivity and specificity of ELISA detection of TB antigen by using a commercially available anti-BCG antibody in conjunction with BACTEC 7H12B culture bottles. We compared these results against those obtained with cultures of Mycobacterium avium-intracellulare and Mycobacterium kansasii. All BACTEC bottles were inoculated with known concentrations of organisms. TB antigen was detected by ELISA in BACTEC culture bottles of TB 12 days before the BACTEC system could itself identify the species. A growth index of greater than or equal to 10 reliably indicated that tuberculosis antigen was detectable by ELISA. The correlation between growth index and antigen concentration was extremely high (r = 0.95 and p less than 0.001). There was insignificant cross-reactivity with the other atypical mycobacteria at the levels of growth tested. This technique potentially offers a sensitive and specific means for the early identification of TB in BACTEC culture bottles.


PMID: 2506783

DOI: 10.1164/ajrccm/140.3.668

Other references

Pela, I.R.; Ferreira, M.E.S.; Melo, M.C.C.; Silva, C.A.A.; Valenzuela, C.F.rnando, 1998: Febrile response induced by platelet-derived growth factor in rats. Kluger, M J [Editor], Bartfai, T [Editor], Dinarello, C A [Editor] Annals of the New York Academy of Sciences; Molecular mechanisms of fever 289-293

Jorgensen, J.H.; Maher, L.A.; Redding, J.S., 1988: Disk diffusion interpretive criteria for extended-spectrum cephalosporins with Haemophilus influenzae. Disk diffusion interpretive zone size criteria for use with Haemophilus test medium are proposed for five extended-spectrum cephalosporins. With cefuroxime, zones greater than or equal to 24 mm in diameter indicated susceptibility, while zones les...

Pathak, D.R.; Samet, J.M.; Humble, C.G.; Skipper, B.J., 1986: Determinants of lung cancer risk in cigarette smokers in New Mexico. Although cigarette smoking is the strongest known risk factor for lung cancer, the effects of specific smoking practices have not been completely characterized. The present study examines determinants of lung cancer risk in a population-based, cas...

Minkin, J.L.; Kellerman, A.S., 1966: A bacteriological method of estimating effectiveness of UV germicidal lamps. Public Health Reports 81(10): 875-884

Huang, X.; Zou, X.; Zhao, J.; Shi, J.; Zhang, X.; Li, Z.; Shen, L., 2015: Sensing the quality parameters of Chinese traditional Yao-meat by using a colorimetric sensor combined with genetic algorithm partial least squares regression. Yao-meat is a traditional Chinese salted meat. Total volatile basic nitrogen content (TVB-N), total viable bacterial count (TVC), and residual nitrite (RN) level are important indexes of freshness for Yao-meat. This paper attempted the feasibility...

Tuckey, R.C.; Stevenson, P.M., 1986: Ferredoxin reductase levels in the ovaries of pigs and superovulated rats during follicular cell growth and luteinization. The concentration of ferredoxin reductase, a component of the mitochondrial steroidogenic electron transport chain, was measured in the ovaries of pigs and superovulated rats by a protein blotting procedure using polyclonal antibodies to the purif...

Garimberti, E.; Tosi, S., 2010: Fluorescence in situ hybridization (FISH), basic principles and methodology. Fluorescence in situ hybridization (FISH) is widely used for the localization of genes and specific genomic regions on target chromosomes, both in metaphase and interphase cells. The applications of FISH are not limited to gene mapping or the stud...

Matsui, D.; Barron, A.; Rieder, M.J., 1996: Assessment of the palatability of antistaphylococcal antibiotics in pediatric volunteers. OBJECTIVE: To evaluate the palatability of four commonly prescribed antistaphylococcal antibiotics, cloxacillin, cephalexin, erythromycin, and fusidic acid, in volunteer children. DESIGN: A single-blind taste test of four flavored antibiotic suspe...

Romussi, G.; Bignardi, G.; Falsone, G.; Wendisch, D., 1987: Constituents of cupuliferae x. triterpene saponins from fagus sylvatica l. From leaves of Fagus silvatica L. in addition to ginsenoside Ro [28-(.beta.-D-glycopyranosyloxy)-28-oxoolean-12-en-3.beta.-yl-2-O-(.beta.-D-glucopyranosyl)-.beta.-D-glucopyranosiduronic acid] (2) a new triterpene saponin has been isolated and iden...

Mittal K.R.; Hartman G.; True L.D., 1987: Immunohistochemical profile of retroperitoneal carcinomas. Laboratory Investigation 56(1): 52A