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Gonadotropin binding sites in eel ovary autoradiographic visualization and new data on specificity

, : Gonadotropin binding sites in eel ovary autoradiographic visualization and new data on specificity. Reproduction Nutrition Developpement 28(4 PART B): 1165-1176

An autoradiographic study of cGTH binding sites in eel ovary indicates that they were mainly located in the follicular layers. Eel ovary membranes exhibited specific 125l-hCG binding in vitro. In control fish we observed no cross-reaction between cGTH and hCG, whatever tracer hormone was used. On the contrary, carp pituitary extract (CPE) inhibited the binding of 125-l-hCG as well as of 125l-cGTH. These results show that a fish pituitary factor, different from the cGTH we used, had binding sites in the ovary. It is suggested that this factor might be an isoform of the gonadotropin. Lastly, we have shown in in vivo CPE-treated eels that cGTH and hCG cross-reacted, demonstrating that the receptor properties changed during CPE-induced gonadal development.


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Dumais, P.; Callender, C.L.; Ledderhof, C.J.; Noad, J.P., 2006: Monolithic integration of microfluidic channels, liquid-core waveguides, and silica waveguides on silicon. The fabrication of embedded microchannels monolithically integrated with optical waveguides by plasma-enhanced chemical vapor deposition of doped silica glass is reported. Both waveguide ridges and template ridges for microchannel formation are pa...