+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Gonadotropin releasing hormone binding sites in human epithelial ovarian carcinomata

, : Gonadotropin releasing hormone binding sites in human epithelial ovarian carcinomata. European Journal of Cancer & Clinical Oncology 25(2): 215-222

As a first step to investigate whether gonadotropin releasing hormone (GnRH) analogs might be able to modulate directly the proliferation of human epithelial ovarian carcinomata, we checked if binding sites for GnRH are present in these malignancies. Specific binding of [125I] [D-Ala6-desGly10]-GnRH-ethylamide (GnRH agonist = GnRH-A) could be demonstrated in plasma membranes from 32 out of 40 ovarian carcinomata tested. This binding was dependent on temperature, time and plasma membrane concentration. Mathematical analysis of the binding data showed that the interaction of GnRH-A with the binding sites was consistent with a single class of low affinity, high capacity binding sites (Ka = 1.42 .+-. 0.14 .times. 105 M-1; range: 0.3-3.8 .times. 105 M-1; R = 209 .+-. 69 .times. 10-12 M/mg membrane protein; range 16-400 .times. 10-12 M/mb MP; .hivin.X .+-. S.E., n = 32). Native GnRH and the GnRH antagonist [D-p-Glu1, D-Phe2, D-Trp3,6]-GnRH had Ka values comparable to those of the GnRH-A used. [125I]GnRH-A binding could not be displaced by oxytocin, thyrotropin releasing hormone and corticotropin releasing factor in concentrations up to 10-4 M. Somatostatin cross-reacted with binding sites from some carcinomata, while it did not displace GnRH-A binding in membranes from others. Though the functional role of this specific binding site for GnRH in human epithelial ovarian carcinomata is still obscure, it might be part of an autocrine regulatory system and provide a possible point of attack for therapeutic approaches using GnRH analogs in this malignancy.


PMID: 2649375

DOI: 10.1016/0277-5379(89)90011-4

Other references

Vladimirova, Aa, 1977: Cottonseed oil cake in unground and ground forms in rations of farm animals and poultry. Dostizheniia mauki i peredovoi opyt v sel'skom khoziaistve Seriia no 2 Zhivotnovodstvo i veterinariia: 25-33

Sancrotti; Iandelli; Olcese; Palenzona, 1991: Si-derived states in Gd silicides via Si L2,3VV Auger line-shape spectroscopy. Physical Review. B, Condensed Matter 44(7): 3328-3331

Sun, L.; Harris, M.E., 2007: Evidence that binding of C5 protein to P RNA enhances ribozyme catalysis by influencing active site metal ion affinity. The RNA subunit ( P RNA) of the bacterial RNase P ribonucleoprotein is a ribozyme that catalyzes the Mg-dependent hydrolysis of pre-tRNA, but it requires an essential protein cofactor ( P protein) in vivo that enhances substrate binding affinitie...

Labonne, M.; Legagneux, B., 1978: Regionalised simulation model of rainfed agriculture in Senegal. The study's three main sections are: I. the model: minimum production units or 'modules'; II. a model of Senegalese agriculture simulating the 1974-75 season; III. use of this model to evaluate critically government objectives 1980-...

Liu, Y.T., 1962: Effect of beryllium and other bivalent cations on some intermediate reactions of glycolysis. Voprosy Meditsinskoi Khimii 8: 392-395

Senapati, S.; Sharma, P.; Bafna, S.; Roy, H.K.; Batra, S.K., 2008: The MUC gene family: their role in the diagnosis and prognosis of gastric cancer. Early diagnosis of gastric cancer and its differential diagnosis from other non-malignant gastric diseases like gastritis is still a major clinical problem. Most patients are asymptomatic in the early stages of gastric cancer, and there is no reli...

Davidović, L.B.; Rancić, Z.S.; Lotina, S.I.; Kostić, D.M.; Marković, D.M.; Pavlović, S.U.; Maksimović, Z.L.; Pejić, M.A.; Jadranin, D.B., 2003: Reconstruction of the supra-aortic branches. The authors present surgical techniques and distant results of the operative treatment in patients with occlusive lesions of the supraaortic branches. The study included 29 men (55.8%) and 23 women (44.2%), with the average age of 54 years. The ma...

Heap, B.J., 1991: Notification of communicable diseases. Bmj 303(6808): 991-991

Simmons, J.Q.; Tymchuk, A.J., 1976: Therapies of mental retardation. Current Psychiatric Therapies 16: 79-89

Szabo, P.; Shen, S.; Telford, W.; Weksler, M.E., 2003: Impaired rearrangement of IgH V to DJ segments in bone marrow Pro-B cells from old mice. There are fewer bone marrow Pre-B cells in old compared to young mice. We have demonstrated both decreased rearrangement of the V to DJ IgH gene segments and low levels of VH germline transcripts in Pro-B cells, the precursors of Pre-B cells, from...