+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Gonadotropin releasing hormone dependent regulation of gonadotropin subunit messenger rna levels in the rat

, : Gonadotropin releasing hormone dependent regulation of gonadotropin subunit messenger rna levels in the rat. Endocrinology 124(1): 272-278

The Nal-Glu GnRH antagonist (GnRHA) was given to castrate male and female rats 7 days after gonadectomy to assess the impact of selective GnRH inhibition on the steady state mRNA level of FSH.beta., LH.beta. and .alpha.-subunit and serum levels of FSH and LH. A low dose of GnRHA (125 .mu.g/kg .cntdot. day) given to female rats for 1, 3, or 7 days resulted in suppression of serum FSH and LH levels by 7 days to 50% and 40%, respectively, of ovariectomized control values. LH.beta. mRNA levels decreased in a time-dependent manner, so that by 7 days, LH.beta. mRNA levels were less than those in intact controls. There were significant but less dramatic declines in .alpha. and FSH.beta. mRNA levels. A higher dose of GnRHA (500 .mu.g/kg .cntdot. day) for 7 or 14 days administered to castrate male or female rats resulted in inhibition of serum LH and FSH to or below levels in intact controls. At this dose, all three gonadotropin subunit mRNA levels fell from castrate values toward or below those in intact controls. Thus, although low dose GnRHA administration supressed LH.beta. mRNA more than FSH.beta. mRNA levels, high dose GnRHA treatment resulted in equal suppression of all three gonadotropin subunits. No stimulatory effects on .alpha.-subunit mRNA levels were observed with either dose of GnRHA. We conclude that the pretranslational control of gonadotropin subunit biosynthesis is GnRH dependent. Adequate dose and length of administration of the potent Nal-Glu GnRHA results in suppression of both the serum gonadotropins FSH and LH and the mRNAs for FSH.beta., LH.beta. and .alpha.-subunit in female and male rats.


Other references

Altmann, D., 1973: Fang und Transport von Halb-und Tieraffen. Unknown

Sikri, G.; Bhattacharya, A., 2016: Novel drugs in the management of acute mountain sickness and high altitude pulmonary edema. Open Access Journal of Sports Medicine 7: 1-3

Schmidt, B.; Weinberg, A.; Friedrich, H., 2008: The mallet finger in children and adolescents. The "mallet finger" in childhood and adolescence differs from the "mallet finger" in adults because of an open or gradually closing epiphysial plate. Thus, our results of conservative and operative treatment were evaluated part...

Nosaka S., 1981: Vagal afferent fiber projections to the dorsal motor nucleus of the vagus nerve. Journal of the Physiological Society of Japan 43(8-9): 310

Igeleke, C.L.; Omorusi, V.I., 2007: Review of post-harvest deterioration of rubber seeds. Journal of Agriculture and Social Research JASR 7(2): 11-19

Pflanz, M., 1961: Epidemiological methods in medical sociology. On a study of the conditions for the spread of disease. Hippokrates 32: 925-933

Duffy, E.A.J., 1946: Observations on Prionus coriarius L. (Col., Cerambycidae) with particular regard to collecting methods. Entomologist's Monthly Magazine, 82: 136-137

Rosenfeld, N.; Ja., 1913: Uber die Wirkung verschiedener Gifte und anderer chemischer Verbindungen auf die Fortpflanzung der Infusorien. Bulletin du Laboratoire Biologique St Peterburg, 132: 62-77

Houston Heidi; Anderson Helen; Zhang Jiajun, 1990: The 20 October 1986 Kermadec earthquake; an anomalous event. Eos, Transactions, American Geophysical Union 71(43): 1468

May, J.R.; Johnson, H.J., 1969: Positive reinforcement and suppression of spontaneous GSR activity. Journal of Experimental Psychology 80(1): 193-195