+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Gonadotropin releasing hormone regulates gonadotropin beta subunit and chromogranin b messenger rna in cultured chromogranin a positive pituitary adenomas

, : Gonadotropin releasing hormone regulates gonadotropin beta subunit and chromogranin b messenger rna in cultured chromogranin a positive pituitary adenomas. Journal of Clinical Endocrinology & Metabolism 71(3): 622-630

Chromogranin-A-positive pituitary adenomas include glycoprotein hormone-producing adenomas, null cell adenomas, and a few other pituitary adenomas. We studied the effects of GnRH, CRF, dexamethasone, and phorbol 12-myristate 13-acetate on FSH and LH secretion and on FSH.beta. and chromogranin-A and -B mRNA expression in 10 chromogranin-A-positive adenomas in vitro to analyze the regulation of FSH and chromogranin-A and -B expression in these neoplasms. Most adenomas responded to GnRH stimulation during 7 days in culture with a 2- to 10-fold increase in FSH and LH secretion and a 2- to 7-fold increase in FSH.beta. mRNA compared to control values. CRF and phorbol 12-myristate 13-acetate also stimulated FSH and LH secretion 2- to 5-fold in five of seven and three of three cases, respectively, during 7 days in culture. Dexamethasone stimulated both FSH and LH secretion in two of three cases as well as FSH.beta. mRNA in vitro in the one case examined. GnRH treamtent consistently produced a 2-fold increase in chromogranin-B mRNA, but not in chromogranin-A mRNA, after 7 days of culture. These results indicate that many chromogranin-A-positive adenomas respond to GnRH and CRF in vitro by increased hormone secretion and that GnRH stimulation leads to increased amounts of FSH.beta. and chromogranin-B mRNAs. The differential response of chromogranin-A and -B mRNAs after GnRH stimulation indicates that the chromogranin genes are highly regulated in these tumors.


Other references

Yuen M.; Huang S.; Sigman Grant M.; Lonnerdal B., 1992: Effects of maternal iron status on iron metabolism in rat mammary gland. FASEB Journal 6(5): A1959

Shepherd, J.P.; Thomas, D.W.; Shepherd, P., 1996: Privatising the NHS: dentistry paves the way. Bmj 312(7036): 922-923

Ikushima, I.; Korogi, Y.; Hirai, T.; Sugahara, T.; Shigematsu, Y.; Okuda, T.; Takahashi, M.; Ushio, Y., 2000: Evaluation of dural sinus invasion and extension of extra-axial intracranial tumors. The advantages of a high-resolution postcontrast 3-D gradient-echo technique. To assess the usefulness of a postcontrast 3-D Fourier transform (3DFT) gradient-echo (GRE) technique in dural sinus invasion and extension of extraaxial intracranial tumors in comparison with a conventional spin-echo (SE) technique. Fourteen cons...

Altaf, A.; Luby, S.; Ahmed, A.J.; Zaidi, N.; Khan, A.J.; Mirza, S.; McCormick, J.; Fisher-Hoch, S., 1998: Outbreak of Crimean-Congo haemorrhagic fever in Quetta, Pakistan: contact tracing and risk assessment. In December 1994 in a private hospital in Quetta, Pakistan, 3 health-workers contracted Crimean-Congo haemorrhagic fever (CCHF) after surgery on a bleeding patient who later died. We conducted a retrospective study to determine transmission risks...

Iordan, M., 1973: Studiul faunei Devonian-Inferioare din dealurile Bujoarele (unitatea M acin-Dobrogea de N). Dari Seam a Sed. Dari de Seama ale Sedintelor Institutul Geologic al Romaniei, 603: 33-70: 1972-

Wingler, A.; Masclaux-Daubresse, Céline.; Fischer, A.M., 2009: Sugars, senescence, and ageing in plants and heterotrophic organisms. Although an involvement of metabolic signals in the regulation of plant senescence has been demonstrated in a range of studies, the exact signalling pathways remain largely unresolved. For leaves, evidence supports a role of sugar accumulation in...

Lee, B.Soo.; Kim, J.Yup.; Park, J.Hun.; Cho, W.Kyung.; Choi, I.S., 2016: Comparative Study on Surface-Initiated ATRP and SI-ARGET ATRP of Oligo(Ethylene Glycol) Methacrylate on Gold. Oligo(ethylene glycol) methacrylate (OEGMA) was polymerized from a polymerization initiator-presenting gold substrate by Activator ReGenerated by Electron Transfer Atom Transfer Radical Polymerization (ARGET ATRP) in water. Compared with the norma...

Nightingale, S.; John, J., 2010: Prospect park-as-lab; place- and inquiry-based geoscience education in an urban high school. The National Science Foundation Teaching Fellows in K-12 Education (GK-12) program, sponsored by Brooklyn College, promotes relationships between scientists in graduate education and inner city public schools. Working with underrepresented minorit...

Gerasimchuk; Gorobets; Goujon-Durand, 1994: Domain-wall motion in antiferromagnets in microwave fields. Physical Review. B, Condensed Matter 49(14): 9608-9612

Nedvaras, A.P.; Marchyulenis, V.I., 1977: Biological and biochemical characteristics of prospective silage plants part 6 content of nutrients in the aboveground part of polygonum weyrichii in the budding flowering and maturing stages and the effect of mineral fertilizers on their accumulation. The content of the nutrient substances was lower in the stems: dry matter, 12.7-21.2; proteins, 9.2-14.8; cellulose, 25.4-41.9; fat, 1.3-2.0; free extractive substances, 39.0-51.5%; ash, 8.6-10.0%; carotene, 1.4-4.5; and ascorbic acid, 6.0-25.8 mg...