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Gonadotropin stimulated protein phosphorylation in porcine granulosa cells


, : Gonadotropin stimulated protein phosphorylation in porcine granulosa cells. Biochemical & Biophysical Research Communications 161(2): 442-447

Treatment of granulosa cells with luteinizing hormone (LH) or follicle-stimulating hormone (FSH) stimulated the phosphorylation of a 58,000 molecular weight protein found in the 900 .times. g pellet. The phosphorylation of this protein was rapid, being significant at 1 min. LH treatment for 30 min induced greater phosphorylation of this protein than did FSH. LH and FSH also appeared to stimulate the phosphorylation of different 900 .times. g pellet proteins. Since both are known to utilize cAMP as a second messenger, the finding of these unique gonadotropin-induced phosphorylations may point to an additional regulatory mechanism.

US$19.90

PMID: 2500116

DOI: 10.1016/0006-291x(89)92618-1


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