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Gram positive bacteria with a high dna g plus c content are characterized by a common insertion within their 23s rrna genes


, : Gram positive bacteria with a high dna g plus c content are characterized by a common insertion within their 23s rrna genes. Journal of General Microbiology 138(6): 1167-1175

An insertion of about 100 bases within the central part of the 23S rRNA genes was found to be a phylogenetic marker for the bacterial line of descent of Gram-positive bacteria with a high DNA G+C content. The insertion was present in 23S rRNA genes of 64 strains representing the major phylogenetic groups of Gram-positive bacteria with a high DNA G+C content, whereas it was found in 23S rRNA genes of 55 (eu)bacteria representing Gram-positive bacteria with a low DNA G+C content and all other known (eu)bacterial phyla. The presence of the insertion could be easily demonstrated by comparative gel electrophoretic analysis of in vitro-amplified 23S rDNA fragments, which contained the insertion. The nucleotide sequences of the amplified fragments were determined and sequence similarities of at least 44% were found. The overall similarity values are lower than those of 16S and 23S rRNA sequences of the particular organism. Northern hybridization experiments indicated the presence of the insertion within the mature 23S rRNA of Corynebacterium glutamicum.

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Other references

Yin, T.H.; Liu, C.M., 1976: Caloric compensation in rats with combined lesions in lateral and ventromedial hypothalamus. Compensatory adjustment of energy intake in response to stomach loading with glucose or vegetable oil was studied in rats with lateral hypothalamic (LH) or with combined lateral and ventromedial hypothalamic (VMH) lesions. The rats with LH lesions...

Bouskela, R.; Chérigié, E.; Jourde, L.; Taieb, A., 1968: Colonic perforations and fistulae. Journal de Radiologie, D'electrologie, et de Medecine Nucleaire 49(11): 876-878

Behrendt, N.; Heegaard, S.; Fornitz, G.G., 1999: The hospital autopsy. An important factor in hospital quality assurance. The frequency of hospital autopsies declined after a new autopsy law was introduced in 1990. The purpose of the study was to evaluate the frequency of autopsy over a six month period in Copenhagen county. We compared the causes of death recorded o...

Fichtenbaum, C.J.; Zackin, R.; Feinberg, J.; Benson, C.; Griffiths, J.K., 2000: Rifabutin but not clarithromycin prevents cryptosporidiosis in persons with advanced HIV infection. Macrolides have been reported to be effective for the prevention of cryptosporidiosis in persons with HIV infection. A cross-protocol analysis was conducted involving 2288 individuals with a history of a CD4 cell counts of <= 100 x 106 cell/lit...

Chavdarov D.; Radionova M., 1990: Eeg mapping spreading and expatiating phenomena. Neurophysiologie Clinique 20(SUPPL): 8S

Kennedy, J.C., 1967: A study of the cellular basis of the primary immune response in mice. Dissertation Abstracts, 27B 3257

Bertoni, A.D.W., 1924: Oeceticus kirbyi in Paraguay. Oeceticus kirbyi, Guild., var. platensis, Berg, is a pest of fruit and other trees in the temperate zone of Argentina, but generally only scattered individuals are seen in Paraguay. Alarming numbers of the moth were, however, observed by the autho...

Aubert, J.S., 1962: Toward research on a treatment of arterial hypertension. Concours Medical 84: 155-166

Tuberosa, R.; Salvi, S.; Phillips, R.L., 1998: Bulked segregant analysis confirms the importance of the region near umc89a for days to pollen shed in maize. F2 populations from the crosses Nt42 x NC254, A679 x NC254 and A86-9 x R225, comprising 520, 350 and 280 plants, respectively, were scored for number of days from planting to pollen shedding (DPS) and selfed. Among the plants which set seed in eac...

Calisher, C.H.; Karabatsos, N.; Lazuick, J.S.; Monath, T.P.; Wolff, K.L., 1988: Reevaluation of the western equine encephalitis antigenic complex of alphaviruses (family Togaviridae) as determined by neutralization tests. Fourteen viruses closely related to the Fleming strain of western equine encephalitis (WEE) virus were cross-tested by serum dilution-plaque reduction neutralization. The results demonstrate that strains McMillan, R-43738, AG80-646, BeAr 102091, a...