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Gram scale purification and preparation of rabbit liver zinc metallothionein

, : Gram scale purification and preparation of rabbit liver zinc metallothionein. Preparative Biochemistry 22(2): 151-164

A simplified procedure for purifying gram quantities of rabbit liver metallothionein (MT) using gel filtration and anion exchange chromatography is presented. The MT purification made use of anion exchange batch elution chromatography which greatly shortened the procedure. Quantitation techniques for use with crude and purified MT are discussed. This paper also describes the preparation of large amounts of ZnMT from Cd, ZnMT.


PMID: 1290494

DOI: 10.1080/10826069208021365

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