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Improved reversed phase high performance liquid chromatographic separation of phosphorus 32 labelled nucleoside 3' 5' bisphosphate adducts of polycyclic aromatic hydrocarbons


, : Improved reversed phase high performance liquid chromatographic separation of phosphorus 32 labelled nucleoside 3' 5' bisphosphate adducts of polycyclic aromatic hydrocarbons. Journal of Chromatography Biomedical Applications 570(1): 65-76

32P-Postlabelling is a sensitive technique for the detection and analysis of carcinogen-DNA adducts. In this paper we describe the development of an improved high-performance liquid chromatography (HPLC) method for the separation of 32P-labelled 3',5'-bisphosphates of nucleosides modified by reactive derivatives of carcinogenic polycylic aromatic hydrocarbons (PAH). Optimal resolution of the major 32P-postlabelled DNA adducts formed by the anti-diol-epoxides of ten PAH was achieved using a phenyl-modified silica gel column with a gradient of methanol in phosphate buffer at low pH and high ionic strength. Use of a radioactivity flow detector coupled to the HPLC apparatus allowed detection of subfemtomole quantities of labelled adducts.

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