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Interaction of carboxyl terminal sequences of human immunodeficiency virus reverse transcriptase with template primer


, : Interaction of carboxyl terminal sequences of human immunodeficiency virus reverse transcriptase with template primer. Journal of Biological Chemistry 266(11): 6774-6779

We have raised a rabbit monospecific antibody (designated C2003) against a synthetic peptide (CTP66) derived from a conserved sequence in the C-terminal portion of the p66 component of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) (DeVico, A.L., Copeland, T. D., Veronese, F.D., Oroszlan, S., Gallo, R. C., and Sarngadharan, M. G. (1989) AIDS Res. Hum. Retroviruses 5, 51-60). This antibody directly inhibits the polymerase activity of HIV-1 RT and of RTs from a variety of retroviruses. HIV-1 RT is protected from this inhibition by preincubation of the enzyme with template primer prior to treatment with the antibody. Such protection is abrogated when the pretreatment is conducted under conditions of high ionic strength. Kinetic studies showed that the antibody-mediated inhibition is competitive with respect to template primer concentration. These results indicate that C2003 antibody acts to interere with the template binding function of the enzyme and further indicates that conserved residues recognized by the antibody may be directly involved in this function.

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Paganetti, H.; Goitein, M., 2000: Radiobiological significance of beamline dependent proton energy distributions in a spread-out Bragg peak. Similar target doses can be achieved with different mixed radiation fields, i.e., particle energy distributions, produced by a practical proton beam and a range modulator. The dose delivered in particle therapy can be described as the integral of...

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Wang, V.L., 1977: Social goals, health policy and the dynamics of development as bases for health education. Among the most powerful social forces in this century is self determination of nations and of people. The relatively recent phenomenon of community participation in health decisions in some countries is but one aspect of the larger societal value....

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Zankai, NP., 1983: Ingestion rates of some Daphnia species in a shallow lake (Lake Balaton, Hungary). Filtering rates of 3 spp. of Daphnia were studied in situ by the 14C technique during the summer in a lake with a water temperature of 20-25 .degree. C (Lake Balaton, Hungary). The renewal time of gut contents proved to be 3-5 min in each of the 3...

Casal Roman M.; Gutierrez Aroca J.; Gonzalez Barranco J.; Ruiz Martinez P., 1985: Study of the in vitro activity of piperacillin against hospital microorganisms. Piperacillin (T-1220) is a new semisynthetic penicillin for parenteral administration, with a broad spectrum of in vitro activity. It is largely eliminated through the kidneys, in active form, and with few side effects. A study was carried out on...

Chen, S.H.ng; Huang, T.C.i; Ho, C.T.ng; Tsai, P.J.n, 1998: Extraction, analysis, and study on the volatiles in roselle tea. The Likens-Nickerson steam distillation procedure was utilized to mimic the preparation of roselle tea. Thermally generated volatiles from roselle were collected and analyzed by GC and GC-MS. There were four differently treated samples: untreated,...

Azzaroli, F.; Mazzella, G.; Montagnani, M.; Festi, D.; Colecchia, A.; Roda, E., 1998: First evidence that UDCA can hasten small bowel transit in humans with gallbladder cholelithiasis. Hepatology 28(4 PART 2): 622A, Oct

Dodge W.S.ott Sr.; Shafer John L.; Klimentidis Robert E., 1996: Capillary pressure; the key to producible porosity. Producible porosity, defined as the pore volume available to hydrocarbon emplacement, has been computed from log measurements by modelling capillary pressure irreducible water saturation as a function of permeability and maximum hydrocarbon column...

Grigorev D.P. (Grigoriev); Iskyul E.V.I.kule H.V., 1937: Differentiation of some silicate melts due to the formation of two immiscible fluids. Acad: URSS, B., Ser. Geol.. 1; Pages 77-106. 1937.