geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Keratin expression in the normal breast and in breast carcinoma


, : Keratin expression in the normal breast and in breast carcinoma. Histopathology (Oxford) 18(6): 517-522

The immunohistochemical reactivities of 69 cases of breast carcinoma were examined on methacarn-fixed, paraffin-embedded sections using eight different monoclonal antibodies which recognize one or a few keratin polypeptides. In the normal breast, the monoclonal antibodies RPN1162, RPN1165 and AE1 stained almost all the luminal cells but not the basal (myoepithelial) cells. The monoclonal antibodies 35BH11, M20, CK5, and CK8.12 stained only a subset of the luminal cells. In contrast, 312C8-1 stained basal cells but not luminal cells. All the tumour specimens reacted with AE1, while over 80% of them also reacted with 35BH11 (57/69), CK5 (57/69) and RPN1165 (55/69); 30% reacted with CK8.12 (21/69) and 16% with RPN1162 (11/69). Based cell-specific keratin, as defined by 312C8-1, was detected in only 1% of cases (1/69). Monoclonal antibodies to different keratin polypeptides may be of use in the characterization and subdivision of breast cancer.

US$19.90

PMID: 1715306

DOI: 10.1111/j.1365-2559.1991.tb01478.x


Other references

Yung, B.Y.M.; Hui, E.K.W.; Chan, P.K., 1992: Protein e23 m.w. pi equals 37 kd 5.1 is the only major protein extracted from hela nucleoli with 3m urea. HeLa nucleoli were isolated using the NP-40 method and subsequently extracted with 3M urea. The extract was incubated at 60.degree. C for 30 min, and precipitated proteins were removed by centrifugation. The supernatant was analyzed by one- and tw...

Zakani, S.; Venne, G.; Smith, E.J.; Bicknell, R.; Ellis, R.E., 2013: Analyzing shoulder translation with navigation technology. Asymmetric stress imposed on the shoulder can lead to anterior shoulder instability in young athletes who perform repetitive overhead motions. A common treatment, surgical anterior capsule tightening, assumes that the instability is caused by abno...

Crichton, R.R.; Ward, R.J., 2004: Iron chelators and their therapeutic potential. Metal Ions in Biological Systems 41: 185-219

Coen, G., 1930: Un, nuovo Lamelli-branco (Limopsis stalioi n. sp.). Atti Acc sci ven -trent-istr Padova 3: 21: 117

Díez, F.; del Hoyo, M.; Serrano, S., 1990: Zidovudine treatment of psoriasis associated with acquired immunodeficiency syndrome. Journal of the American Academy of Dermatology 22(1): 146-147

Cottin, H.; Coll, P.; Coscia, D.; Fray, N.; Guan, Y.Y.; Macari, F.; Raulin, F.; Rivron, C.; Stalport, F.; Szopa, C.; Chaput, D.; Viso, M.; Bertrand, M.; Chabin, A.; Thirkell, L.; Westall, F.; Brack, A., 2008: Heterogeneous solid/gas chemistry of organic compounds related to comets, meteorites, Titan, and Mars; laboratory and in lower Earth orbit experiments. To understand the evolution of organic molecules involved in extraterrestrial environments and with exobiological implications, many experimental programs in the laboratory are devoted to photochemical studies in the gaseous phase as well as in th...

Li, W-Hao.; He, Y., 2007: Research progress of microparticles as drug delivery system for traditional Chinese medicine. Microparticle preparation, a new drug delivery system based on microencapsulation technique, includes micro-spheres and microcapsules. Recently, this new drug delivery system has been applied in developing new dosage forms for the traditional Chin...

Burret, F.C.ulia, A.; Debelmas, A.; Hostettmann, K., 1978: Presence of isoscoparine, isoscoparine-7-O-glucoside and Saponarine in gentiana pneumonanthe L. Planta medica 34(2): 176-179

Kelly, C.F.; Bond, T.E.; Ittner, N.R., 1955: Water cooling for livestock in hot climates. Trials were made from 1947 to 1954 to show the value of different systems of cooling for beef cattle in a hot climate. Cooling by spraying the animals, by cooling the air by evaporation in shelters enclosed on 3 sides, by cooling the roofs of open...

Zhang, L.; You, J.; Ping, G.; Zhang, W.; Yan, C.; Zhang, Y., 2005: Micellar electrokinetic capillary chromatographic separation of amino acids derivatized with 2-(9-carbazole)-ethyl chloroformate as a pre-column labeling reagent. 2-(9-Carbazole)-ethyl chloroformate (CEOC) has been used for the pre-column derivatization of amino acids in micellar electrokinetic capillary chromatography (MEKC). Nine amino acid standards (Ser, Thr, Gly, Glu, Asp, Val, Ile, Leu, Phe) were labe...