geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Kinetic and molecular parameters of human hepatic porphobilinogen deaminase


, : Kinetic and molecular parameters of human hepatic porphobilinogen deaminase. Biochemistry International 21(3): 463-471

A basal level of human liver porphobilinogen deaminase of 3.66 units/g wet weight was found in adult tissue. Activity in neonatal liver was at least three fold higher. Physico-chemical studies revealed that the enzyme has the approximate form of a symmetrical molecule and exhibits hyperbolic kinetics with a Km value of 3.6 microM at pH 7.6. Two ionizable groups with pK values of 7.35 and 8.90 are prominent for catalysis. A set of pI (5.8-4.9) were observed under different conditions. Results demonstrate the existence of a single protein differentially charged with multiple molecular forms in adult liver.

US$29.90

PMID: 2222491


Other references

Marin, M.A.; Ziburkus, J.; Jankowsky, J.; Rasband, M.N., 2016: Amyloid-β plaques disrupt axon initial segments. Amyloid-β (Aβ) plaques are one of the central pathologies of Alzheimer's disease (AD). Plaque formation in animal models of AD coincides with the appearance of synaptic abnormalities, aberrant neuronal excitability, and cognitive decline. A...

Roscoe J.M.; Danielpour D.; Riss T.L.; Ogasawara M.; Sirbasku D.A., 1986: Growth of mtw 9 pl2 rat mammary tumor cells in serum free hormonally defined medium. Journal of Cell Biology 103(5 PART 2): 14A

Reedijk, M.F.; Arsic, J.; Kaminski, D.; Poodt, P.; Van Kessel, J.W.M.; Szweryn, W.J.; Knops, H.; Vlieg, E., 2003: New (sqrt3 xsqrt 3)R30 degrees phase of Pb on Ge(111) and its consequence for the melting transition. Depending on the preparation method, we find two different structures of the Pb/Ge(111) system at a nominal coverage of 4 / 3 monolayer that exhibit different melting points. One is the well studied beta phase that melts at 270 degrees C, but the...

Wu, M.; Han, F.; Gong, W.; Feng, L.; Han, J., 2016: The effect of copper from water and food: changes of serum nonceruloplasmin copper and brain's amyloid-beta in mice. Copper is an essential element and also produces adverse health consequences when overloaded. Food and water are the main sources of copper intake, however few studies have been conducted to investigate the difference between the ways of its intak...

Van Slobbe W.G., 1983: Control of castnia daedalus a major pest of oil palm elaeis guineensis in surinam. C. daedalus Cramer, a lepidopterous trunk borer in oil palm, is commonly controlled by spraying endrin, monocrotophos, dipeterex and sevin. Experiments to kill the larvae by injecting insecticides (Monocrotophos and Furadan) into the trunk were ca...

Giannina, G.; Moise, K.J.; Dorman, K., 1998: A simple method to estimate volume for fetal intravascular transfusions. We derived a constant termed the transfusion coefficient to simplify the estimation of the fetal intravascular transfusion volume. The product of the estimated fetal weight (g) and 0.02 (transfusion coefficient), estimates the transfusion volume (...

Ghaemi, S.Nassir.; Hsu, D.J.; Rosenquist, K.J.; Katzow, J.J.; Goodwin, F.K., 2004: Long-term observational comparison of risperidone and olanzapine in bipolar disorder. To compare long-term effectiveness and safety of risperidone versus olanzapine as adjunctive maintenance treatments of bipolar disorder. Retrospective observational chart review of 29 outpatients with bipolar or schizoaffective disorder (type I =...

Yu, J.; Liu, F.; Yin, P.; Zhu, X.; Cheng, G.; Wang, N.; Lu, A.; Luan, W.; Zhang, N.; Li, J.; Guo, K.; Yin, Y.; Wang, H.; Xu, J., 2011: Integrating miRNA and mRNA expression profiles in response to heat stress-induced injury in rat small intestine. The molecular mechanisms underlying the pathophysiology of heat stress in the small intestine remain undefined. Furthermore, little information is available concerning changes in microRNA (miRNA) expression following heat stress. The present study...

Skvorak, J.P.; Waugh, J.D.; Miller, T.L.; Snider, T.H., 1999: Nerve agent penetration through porcine and human skin and efficacy of a fielded decontaminant. FASEB Journal 13(4 PART 1): A155, March 12

Anonymous, 1925: Public Health Weekly Reports for JANUARY 16, 1925. Public Health Reports 40(3): 105-137