+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Low temperature spectroscopy of dna intercalator ethidium

, : Low temperature spectroscopy of dna intercalator ethidium. Journal of Luminescence 43(1): 41-50

The fluorescence spectrum of ethidium, a well-known dye that binds DNA by intercalation, is interpreted in terms of vibronic components. The role of solvents such as water, ethanol and glycerol together with that of the host DNA is examined and the parameters of the linear electron-vibration interactions are estimated. Hard mode frequencies of 1200-1300 cm-1 and soft mode frequencies of 200-300 cm-1 are obtained. A description of the thermal dependence of the ethidium fluorescence quantum yield in different media between 100 and 350 K is attempted in terms of the thermal activation to a radiationless level.


Other references

Seri, I.; Rudas, G.; Bors, Z.; Kanyicska, B.; Tulassay, T., 1993: Effects of low-dose dopamine infusion on cardiovascular and renal functions, cerebral blood flow, and plasma catecholamine levels in sick preterm neonates. Effects of 2 and 4 mu-g/kg/min dopamine infusion on cardiovascular and renal functions, cerebral blood flow (CBF) and plasma catecholamine levels were studied in sick preterm neonates during the first four days of life. Preterm infants were found...

Arroyo, M.T.K.; Dudley, L.S.; Jespersen, G.; Pacheco, D.A.; Cavieres, L.A., 2014: Temperature-driven flower longevity in a high-alpine species of Oxalis influences reproductive assurance. How high-alpine plants confront stochastic conditions for animal pollination is a critical question. We investigated the effect of temperature on potential flower longevity (FL) measured in pollinator-excluded flowers and actual FL measured in pol...

Wermelinger, B.; Delucchi, V., 1990: Effect of sex-ratio on multiplication of the two-spotted spider mite as affected by leaf nitrogen. Spider mites (Tetranychus urticae) were reared individually on leaf discs of apple trees fertilized with three different N levels. Leaf nitrogen, female fecundity and mortality as well as offspring sexratio were determined. The sex-ratio and fecun...

Kono, N.; Ohto, U.; Hiramatsu, T.; Urabe, M.; Uchida, Y.; Satow, Y.; Arai, H., 2013: Impaired α-TTP-PIPs interaction underlies familial vitamin E deficiency. α-Tocopherol (vitamin E) transfer protein (α-TTP) regulates the secretion of α-tocopherol from liver cells. Missense mutations of some arginine residues at the surface of α-TTP cause severe vitamin E deficiency in humans, but the role of these...

Kulikov, G.V., 1971: Broad-leaved evergreen trees and shrubs. The biological, ecological and ornamental properties are described of 57 broad-leaved evergreen species for landscape planting in the south of the USSR. Special attention is paid to drought resistance, winter hardiness and propagation methods.

Keefer, L.K.; Johnson, D.E., 1972: Magnesium hydroxide as a thin-layer chromatographic adsorbent. 3. Application to separations of vitamin A and related carotenoids. 3. Chromatography on Mg(OH)2 with CS2 as solvent was used to separate alpha - and beta -carotene and lycopene, also retinol, retinaldehyde and retinyl acetate.

Anklesaria P.; Pratt D., 1992: Expression of selectins on human cd34 positive progenitor cells and their involvement in mediating adhesion of stem cells to marrow stroma. Journal of Cellular Biochemistry Supplement (16 PART C): 94

Sakaguchi, Yuzi, 1963: Studies on ancylostomiasis in the farm district 2 Seasonal observation of survival of hookworm ova in the privy pits and outside soil-reservoirs. Seasonal observations about the growth and survivability of hookworm eggs stored in privy pits and outside soil-reservoirs were made practically and experimentally. Samples were taken at given intervals in all seasons for examination. Their hatcha...

Michel, P.A.; Kase, J.A., 2009: Genetic profiles of Shiga toxin and intimin genes found in stool broth cultures: a 2-year reference laboratory study. Shiga toxin (Stx)-producing Escherichia coli (STEC) are associated with potentially serious illness in humans. STEC detection is often based on the presence of Stxs, Stx(1) and/or Stx(2), and intimin, encoded by the eae gene. A 2-year collection o...

Tully, S., 1993: Why drug prices will go lower. The industry's days of gargantuan earnings are gone forever. Why? Managed-care outfits are demanding price breaks, and generics are proving tough competitors.