+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Mapping of the high affinity Fc epsilon receptor binding site to the third constant region domain of IgE

, : Mapping of the high affinity Fc epsilon receptor binding site to the third constant region domain of IgE. Embo Journal 10(1): 101-107

Identification of the precise region(s) on the IgE molecule that take part in the binding of IgE to its high affinity receptor (Fc.epsilon.RI) may lead to the design of IgE analogues able to block the allergic response. To localize the Fc.epsilon.RI-binding domain of mouse IgE, we attempted to confer on human IgE, which normally does not bind to the rodent receptor, the ability to bind to the rat Fc.epsilon.RI. Employing exon shuffling, we have expressed chimeric .epsilon.-heavy chain genes composed of a mouse (4-hydroxy-3-nitrophenyl)acetic acid (NP)-binding VH domain, and human C.epsilon. in which various domains were replaced by their murine counterparts. This has enabled us to test the Fc.epsilon.RI-binding of each mouse IgE domain while maintaining the overall conformation of the molecule. All of the chimeric IgE molecules which contain the murine C.epsilon.3, bound equally to both the rodent and human receptor, as well as to monoclonal antibodies recognizing a site on IgE which is identical or very close to the Fc.epsilon.RI binding site. Deletion of the second constant region domain did not impair either the binding capacity of the mutated IgE or its ability to mediate mast cell degradation. These results assign the third epsilon domain of IgE as the principal region involved in the interaction with the Fc.epsilon.RI.


PMID: 1824934

Other references

Quintavalle, M.; Sambucini, S.; D.P.etro, C., 2006: The a Isoform of Protein Kinase CKI Is Responsible for Hepatitis C Virus NS5A Hyperphosphorylation. Journal of Virology 80(22): 305-12

Kwekkeboom, J.; Bruyneel, B.; Tilanus, H.W.; Ijzermans, J.N.M.; Bouma, G.J.; Zondervan, P.E.; Metselaar, H.J., 2000: Are donor-livers with low expression of CD80 at the time of transplantation less susceptible to acute rejection?. Journal of Hepatology 32(Supplement 2): 61

Dijkhuis, J.J., 1951: Theoretical remarks on movement therapy. Folia Psychiatrica, Neurologica et Neurochirurgica Neerlandica 54(1): 49-57

Tatarintsev V.I.; Sandomirskaya S.M.; Tsymbal S.N., 1987: First discovery of titanium nitride (osbornite) in terrestrial rocks. Doklady Akademii Nauk SSSR 296(6): 1458-1461

Mehta, S.K.; Tucci, M.A.; Benghuzzi, H.A., 2013: Effect of extracellular adenosine triphosphate on activity of osteoblast like cells - biomed 2013. Platelet dense granules contain serotonin, adenosine triphosphate (ATP), and adenosine diphosphate (ADP). These molecules are present in platelet rich plasma (PRP), and may therefore have an impact on the efficacy of PRP therapy. Additionally, nuc...

Shoenfelt C.E., 1937: Oil and gas development in Colorado in 1936. AI

Uralov M., 1966: Some physicochemical factors affecting the evagination of cysticercus bovis in vitro. Nauch Tr Samarkand Univ (156): 137-141

Andrysek, T.; Stuchlik, M.; Matha, V.; Jegorov, A.; Husek, A.; Stuchlik, J., 2002: Immunomodulatory pharmaceutical composition adopted to form lyotrophic liquid crystals in contact with an aqueous phase. Pharmaceutical compositions especially for internal application which create lyotropic liquid crystals when in contact with aqueous phase. The pharmaceutical compositions consist of: A hydrophobic active ingredient with immunomodulatory effect fro...

Petrov, L.; Cucuianu, A.; Bojan, A.; Vasilache, A., 1998: Prognostic factors in gastrointestinal non-Hodgkins lymphomas. British Journal of Haematology 102(1): 200-201, July 1

Rintala, J.; Jaatinen, P.; Parkkila, S.; Sarviharju, M.; Kiianmaa, K.; Hervonen, A.; Niemelä, O., 2000: Evidence of acetaldehyde-protein adduct formation in rat brain after lifelong consumption of ethanol. Acetaldehyde, the first metabolite of ethanol, has been shown to be capable of binding covalently to liver proteins in vivo, which may be responsible for a variety of toxic effects of ethanol. Acetaldehyde-protein adducts have previously been dete...