+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Monoclonal antibodies facilitate analyses of ocular development in mice

, : Monoclonal antibodies facilitate analyses of ocular development in mice. Experimental Eye Research 47(4): 555-564

To clarify molecular fluctuations during ocular development, we prepared monoclonal antibodies named MAb 111 and MAb 23 which bound differentially to the neural retina, pigmented epithelium, iris and ciliary body. In newborn mice, MAb 111 bound to the pigmented epithelium including the retinal pigmented epithelium, the pigmented layer of the ciliary epithelium and the outer layer of the iris epithelium. MAb 111 also bound to the non-pigmented layer of the ciliary epithelium and the inner layer of the iris epithelium. MAb 23 bound to the neural retina, the pigmented layer of the ciliary epithelium and the outer layer of the iris epithelium. Using these two monoclonal antibodies, we performed immunohistochemical analyses on developing mouse eyes and reached the conclusion that the optic cup has already been determined to differentiate into the retina, ciliary body and iris, on the 15th day of gestation, when the ciliary body and iris are not morphologically demarcated.


PMID: 3181334

DOI: 10.1016/0014-4835(88)90094-2

Other references

Scott, H.M.; Soskolne, C.L.; Martin, S.W.; Basarab, J.A.; Coppock, R.W.; Guidotti, T.L.; Lissemore, K.D., 2003: Lack of associations between air emissions from sour-gas processing plants and beef cow-calf herd health and productivity in Alberta, Canada. This paper describes a large-scale investigation into the effects of licensed air emissions from sour-gas processing plants on the health and productivity of beef cow-calf herds in the province of Alberta, Canada. In conjunction with a geographica...

Segura Castruita, M.A.; Gutierrez Castorena, M. del C.; Ortiz Solorio, C.A.; Sanchez Guzman, P., 2005: Moisture regime and classification of pumiceous soils of Puebla-Tlaxcala Valley. Pumice soils have high holding capacity because of the presence of porous particles of 'lapilli' size (pumice); this capacity may influence the moisture regime (Mr) and, therefore their technical classification. The objectives were to es...

Lipscomb, P.R.; Wakim, K.G., 1961: Further observations in the healing of severed tendons: an experimental study. Proceedings of the Staff Meetings. Mayo Clinic 36: 277-282

Shiose, Y., 1968: Histochemistry of dehydrogenases in the ciliary epithelium. Nagoya Medical Journal 14(4): 299-315

Deckwerth, Thomas L., 1998: Molecular mechanisms of neuroprotection from neuronal death by trophic factor deprivation. Mattson, M P [Editor] Contemporary Neuroscience; Neuroprotective signal transduction 61-82

Long J.B., 1988: Spinal subarachnoid injection of somatostatin causes neurological deficits and neuronal injury in rats. The tetradecapeptide somatostatin produced dose-related neurological deficits following subarachnoid injection ir the lumbar spinal cords of rats. Lower pharmacological doses (1.6 and 3.1 nmol, i.t.) of somatostatin caused only transient deficits,...

Srinivas, T.R.; Meier-Kriesche, H-Ulf., 2006: Obesity and kidney transplantation. Obesity is an increasingly common comorbidity at the time of kidney transplantation. Obesity is also a frequent complication of kidney transplantation. The obese transplant recipient suffers adverse metabolic and cardiovascular consequences of obe...

Perras, J.G., 1971: The presence of Quercus borealis in the county of Charlevoix, Quebec. The presence of some stunted trees in mixed coniferous forest on slopes (ca. 850 ft alt.) surrounding Lake Port-aux-Quilles gives a point of reference for the extreme NE limit of distribution (69 degrees 50'W. and 47 degrees 50'N.) of Q....

Hasinoff, B.B., 1984: Kinetics of carbonic anhydrase catalysis in solvents of increased viscosity: a partially diffusion-controlled reaction. Steady-state kinetic studies of the bovine carbonic anhydrase B-catalyzed hydration of CO2, dehydration of HCO3-, and hydrolysis of p-nitrophenylacetate were made in glycerol/water solvents of increased viscosity in order that the effect of diffus...

Gooi, J.H.; Pompolo, S.; Karsdal, M.A.; Kulkarni, N.H.; Kalajzic, I.; McAhren, S.H.M.; Han, B.; Onyia, J.E.; Ho, P.W.M.; Gillespie, M.T.; Walsh, N.C.; Chia, L.Y.; Quinn, J.M.W.; Martin, T.J.; Sims, N.A., 2010: Calcitonin impairs the anabolic effect of PTH in young rats and stimulates expression of sclerostin by osteocytes. The therapeutic goal of increasing bone mass by co-treatment of parathyroid hormone (PTH) and an osteoclast inhibitor has been complicated by the undefined contribution of osteoclasts to the anabolic activity of PTH. To determine whether active os...