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On line connector for microcolumns application to the on column o phthaldialdehyde derivatization of amino acids separated by capillary zone electrophoresis

, : On line connector for microcolumns application to the on column o phthaldialdehyde derivatization of amino acids separated by capillary zone electrophoresis. Analytical Chemistry 60(23): 2625-2629

The design of a simple, on-column connector for microcolumn separations is described. The connector, in the form of a cross or tee, is fabricated from the fused silica capillary tubing, itself, and has sufficiently low dead volume to be compatible with the tubing dimensions normally associated with microcolumn separation techniques. As a demonstration of one possible application, a mixture of amino acids is separated by capillary zone electrophoresis (CZE), the amino acids are derivatized on-column with 0-phthaldialdehyde (OPA) introduced from a cross connector, and the highly fluorescent adducts are detected downstream by laser-induced fluorescence. Zone broadening by the connector is determined to be approximately 10% for CZE separations performed in 75 .mu.m i.d. capillary tubes. Detector response is found to be linear over more than 3 orders of magnitude with minimum limits of detection in the subfemtomole range.


PMID: 3239811

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