+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Permanent dipole moment of transfer rna and variation of their structure in solution

, : Permanent dipole moment of transfer rna and variation of their structure in solution. Biophysical Journal 58(2): 403-412

The structure of six different tRNA molecules has been analyzed in solution by electrooptical measurements and by bead model simulations. The electric dichroism measured as a function of the field strength shows that tRNA's are associated with substantial permanent dipole moments, which are in the range of 1 .cntdot. 10-27 cm(.tbd. 300 D; before correction for the internal directing field). Rotational diffusion time constants of tRNA molecules in their native state at show a considerable variation. A particularly large value found for tRNATyr (50 ns) can be explained by its nine additional nucleotide residues. However, remarkable variations remain for tRNA molecules with the standard number of 76 nucleotide residues (tRNAPhe [yeast] 41.6 ns, tRNAVal [Escherichia coli] 44.9 ns, tRNAGlu [E. coli] 46.8 ns; tRNAPhe [E. coli] 48.3 ns). These variations indicate modulations of the tertiary structure, which may be due to a change of the L-hinge angle. Bead models are used to simulate both electric and hydrodynamic parameters of tRNA molecules according to the crystal structure of tRNAPhe (yeast). The asymmetric distribution of phosphate charges with respect to the center of diffusion leads, under the assumption of a constant charge reduction to 15% by ion condensation, to a theoretical dipole moment of 7.2 .cntdot. 10-28 cm, which is in reasonable agreement with the measurements. The dichroism decay curve calculated for tRNAPhe (yeast) is also consistent with the measurements and thus the structure in solution and in the crystal must be very similar in this case. However, our measurements also indicate that the structure of some other tRNA's in solution is different, even in cases with the same number of nucleotide residues.


Other references

Allen J.R.; Carstens L.A., 1968: Response of saimiri sciureus monkeys to inst total body x irradiation clinical signs and pathologic changes. American Journal of Veterinary Research 29(11): 2179-2188

Goyzhevskiy A.A., 1968: The Volchansk projection of the Ukrainian shield. East-west and north-south fracture zones, Eocene deposits in depressions, recent positive block movements, USSR.

Trotter, A.; Maier, L.; Grill, H.J.; Wudy, S.A.; Pohlandt, F., 1999: 17Beta-estradiol and progesterone supplementation in extremely low-birth-weight infants. During pregnancy, 17beta-estradiol (E2) and progesterone (P) plasma concentrations increase up to 100-fold. The fetus is exposed to these increasing amounts of E2 and P. Within 1 d after delivery, E2 and P concentrations fall to nonpregnancy conce...

North E., 1979: Aggressive behavior of juvenile brown trout salmo trutta an analysis of the wigwag display. The wigwag display of the juvenile brown trout (S. trutta, L) was analyzed and a ritualized display used specifically as a defensive maneuver in the aggressive interactions of groups of fish was found. Wigwag displays performed against fish of low...

Larsen, M.H., 1982: Dry cow therapy and its impact on the frequency of bovine mastitis. Detailed observations of a herd of 56 cows treated when dry between October 1979 and June 1980, and 37 cows, only 17 of which received antibiotic, between October 1980 and June 1981, led to the conclusion that this form of antibiotic therapy did n...

Lee E.; Horiguchi M., 1991: Effects of feed rate on the microbiological and chemical characteristics of rumen microbial fermentation of rice straw in the continuous culture system. The purpose of this study was to examine the effects of feed rate at a constant liquid dilution rate on chemical and microbiological changes in rumen microbial fermentation of rice straw in a continuous culture system. Rice straw powder of 0.5 mm...

Crisalli, M.; Scarabicchi, S., 1957: Coxsackie virus diseases; case reports. Minerva Pediatrica 9(21-22): 593-605

Perl, A.; Gonzalez-Cabello, R.; Láng, I.; Gergely, P., 1982: Depressed natural and lectin-dependent cell-mediated cytotoxicity against adherent HEp-2 cells in patients with systemic lupus erythematosus. Natural cell-mediated cytotoxicity /NCMC/ was evaluated using human adherent 3H-thymidine-prelabelled HEp-2 epipharynx carcinoma cells as targets at 50:1 effector-target cell ratio in a 24 hr assay. For lectin-dependent cell-mediated cytotoxicity...

Dutau, G., 1988: Allergic field: how to detect it?. La Revue du Praticien 38(20): 1341-1345

Confavreux, C., 2004: Intravenous immunoglobulins, pregnancy and multiple sclerosis. Acute exacerbations may complicate the course of pregnancy and the postpartum period in patients with relapsing-remitting multiple sclerosis (RRMS). To evaluate relapse rate and the effect of immunomodulatory treatment with intravenous immunoglobu...