+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Possibility of incorporation of macromolecules including exogenous dna into sex cells the liposome method and calcium phosphorus coprecipitation method

, : Possibility of incorporation of macromolecules including exogenous dna into sex cells the liposome method and calcium phosphorus coprecipitation method. Tsitologiya i Genetika 24(2): 52-55

Liposomes loaded with FITC-labeled albumin in the presence of PEG-1 500 are actively sorbed on the membranes of mature spermatozoa and remain attached even after thorough washing. Immature sperm cells are able to incorporate alien DNA carried by liposomes. In contrast, the mature spermatozoa could not incorporate plasmid DNA loaded with positively charged liposomes. Chlortetracycline in Ca-P coprecipitate crystals is tightly fixed in the postacrosomal region of mature sperms. Intensity of staining of chlortetracycline is stimulated by DNA load in Ca-P coprecipitate as well as by DMSO or EDTA. The method of Ca-P coprecipitation could not provide for plasmid DNA incorporation into taure sperms. Foreign DNA incorporation in postacrosomal regions of sperm heads seems quite possible in experiments with dimethylsulphozide (DMSO).


Other references

Sugiyama, S., 1954: Studies of the histogenesis of the thyroid gland of the guinea pig. I. The thyroid cells (follicle cells and parafollicular cells). Anatomical Record 120(2): 363-377

Wang, Z.; Chen, X.; Zhou, L.; Wu, D.; Che, X.; Yang, G., 2003: Effects of extracellular signal-regulated kinase (ERK) on focal cerebral ischemia. Objective To determine the role of extracellular signal-regulated kinase (ERK) 1/2 during focal cerebral ischemia. Methods Left middle cerebral artery occlusion (MCAO) was undergone after the introduction of a nylon suture to the left internal car...

Nicolò, F.; Costetti, A.; Carro, C.; Marianeschi, S.Maria., 2016: Off-pump Kommerell's diverticulum resection and descending aorta replacement. We report the case of an unusual variation of a Kommerell's diverticulum in a left aortic arch with an aberrant left subclavian artery, associated with dilatation of the distal aortic arch, surgically treated without the use of extracorporeal...

Sadacca, L.A.; Lamia, K.A.; deLemos, A.S.; Blum, B.; Weitz, C.J., 2011: An intrinsic circadian clock of the pancreas is required for normal insulin release and glucose homeostasis in mice. Loss of circadian clocks from all tissues causes defective glucose homeostasis as well as loss of feeding and activity rhythms. Little is known about peripheral tissue clocks, so we tested the hypothesis that an intrinsic circadian clock of the pa...

Morimoto, T.; Higaki, T.; Ota, M.; Inawaka, K.; Kawamura, S.; Bungo, T., 2014: Effect of simultaneous exposure to mixture of two skin sensitizers on skin sensitization response in guinea pigs and mice. Skin sensitization resulting in allergic contact dermatitis is a common occupational health issue. In this study, the effect of mixing two skin sensitizers on the skin sensitization response was investigated. Skin sensitizers are generally classif...

Wiseman, R.D.; Wiseman, J.R., 1964: Value Of Home Visits In The Treatment Of The Allergic Patient. New York State Journal of Medicine 64: 1948-1951

Villa, J.C.; Chiu, D.; Brandes, A.H.; Escorcia, F.E.; Villa, C.H.; Maguire, W.F.; Hu, C-Jun.; de Stanchina, E.; Simon, M.Celeste.; Sisodia, S.S.; Scheinberg, D.A.; Li, Y-Ming., 2015: Nontranscriptional role of Hif-1α in activation of γ-secretase and notch signaling in breast cancer. γ-Secretase is composed of four proteins that are obligatory for protease activity: presenilin, nicastrin, Aph1, and Pen-2. Despite the progress toward understanding the function of these individual subunits, there is no information available per...

Pollack A.; Prudhomme D.; Irvin G.L.IIi; Claflin A.J.; Block N.L., 1981: Flow cytometric analysis of rna content per cell using pyronin y and methyl green. Cytometry 2(2): 122

Maggi, A.; Michelini, A., 2009: Rapid waveform earthquake location in Italy. Geophysical Research Abstracts 11

Fernandes, A.; Wojcik, T.; Baireddy, P.; Pieschl, R.; Newton, A.; Tian, Y.; Hong, Y.; Bristow, L.; Li, Y-Wen., 2016: Inhibition of in vivo [(3)H]MK-801 binding by NMDA receptor open channel blockers and GluN2B antagonists in rats and mice. N-methyl-D-aspartate (NMDA) receptor antagonists, including open channel blockers and GluN2B receptor subtype selective antagonists, have been developed for the treatment of depression. The current study investigated effects of systemically admini...