+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Proliferative capacity of human peripheral blood lymphocytes sorted on the basis of glutathione content

, : Proliferative capacity of human peripheral blood lymphocytes sorted on the basis of glutathione content. Journal of Cellular Physiology 145(3): 472-480

Glutathione (GSH) is important in defense against oxygen free radical damage, in detoxification of xenobiotics, and in mitogenesis. The reducing conditions provided by low molecular weight thiols such as 2-mercaptoethanol (ME) have been shown to promote the growth of lymphocytes in culture. We wished to determine the effects of 2-ME on GSH content, and to determine to what extent GSH status affected lymphocyte proliferation. GSH content was quantitated in human peripheral blood lymphocytes (PBL) using a flow cytometric assay with monochlorobimane. This analysis was performed on PBL as well as on the CD4+ T-cell subset, as identified with fluorescent anti-CD4 monoclonal antibodies (mAb). Cells were viably sorted on the basis of their GSH content, and incubated for 3 days with mitogenic concentrations of PHA (for PBL) or anti-CD3 mAb (for CD4+ cells) in the presence of bromodeoxyuridine (BrdU). BrdU/Hoechst cell cycle analysis was then performed on these cells. High GSH sorted cells had a higher percentage of cells capable of entering the cell cycle than low GSH sorted cells. This data indicates that some of the heterogeneity in proliferative capacity within PBL in culture is directly or indirectly related to GSH content. Incubation of cells in 2-ME prevented the loss of GSH that occurs when cells are cultured. 2-ME improved the proliferative capacity of unsorted cells, and of cells sorted for high and low GSH. Acridine orange staining of anti-CD3 mAb stimulated cells sorted for high and low GSH indicated that an early event in cell activation was affected by GSH content.


PMID: 1703168

DOI: 10.1002/jcp.1041450312

Other references

Skrzypczak L.; Wesolowska M.; Thiem B., 1988: Callus culture and micropropagation of eustoma grandiflorum shinn. Regenerated plants and callus tissue were obtained by embryo culture and micropropagation of explants from shoots and leaves of Eustoma grandiflorum Shinn. (Gentianaceae), a medicinal plant. This species, which does not naturally occur in this cou...

Howell, Arthur H., 1907: The retation of birds to the cotton boll weevil. U S Department of Agriculture Biological Survey Bulletin, 29 1-31

Huang, H.; Xu, D.; Liu, M.; Jiang, R.; Mao, L.; Huang, Q.; Wan, Q.; Wen, Y.; Zhang, X.; Wei, Y., 2017: Direct encapsulation of AIE-active dye with β cyclodextrin terminated polymers: Self-assembly and biological imaging. Aggregation-induced emission (AIE) phenomenon has attracted great attention recently and been extensively explored for biomedical applications. Nevertheless, the direct utilization of AIE-active dyes for biomedical applications has demonstrated to...

Ellis, G.K.; Crowley, J.; Livingston, R.B.; Goodwin, J.W.; Hutchins, L.; Allen, A., 1991: Cisplatin and novobiocin in the treatment of non-small cell lung cancer. A Southwest Oncology Group study. Novobiocin, a commercially available oral antibiotic, inhibits DNA topoisomerase II in a manner shown in cell culture to enhance the cytotoxicity of alkylating agents and cisplatin. Thirty-six patients were entered on a Phase II trial using high-d...

Kolmer, J.A.; Brown, H.; Rule, A.M., 1941: The therapeutic activity of the organic arsenical compounds in syphilis of rabbits in relation to the urinary excretion of arsenic. Single intraven. injns. of arsphena-mine in dose of 0.012 g. per kg.; of neoarsphenamine in dose of 0.016 g.; of sulfarsphenamine (intramusc.) in dose of 0.024 g.; of bismarsen (intramusc.) in dose of 0.012 g. all resulted in the biologic cure of...

Woźniak, W.; Young, P.A., 1968: Nerve cells in the extracranial portion of the hypoglossal nerve in human fetuses. The hypoglossal nerve with its communicating branches between the hypoglossal canal and its terminal rami was removed in toto from 11 human fetuses varying from 63 to 342 mm C.R. length. Serial sections were stained with luxol fast blue or impregn...

Wolff T.; Hesse S., 1977: Different metabolic rates of a chloro bi phenyl in liver microsomes after pre treatment of rabbits with poly chlorinated bi phenyls 3 methyl cholanthrene and pheno barbital. INT UNION PHARMACOL PROCEEDINGS OF THE INTERNATIONAL CONGRESS OF PHARMACOLOGY, 6TH ABSTRACT HELSINKI, FINLAND, 1975 632p ILLUS PERGAMON PRESS: OXFORD, ENGL ; NEW YORK, N Y , USA ISBN 0-08-021308-1 577

Del Prior, G.; Lurain, J.R., 1993: The ability of Papanicolaou smears and colposcopy to the predict the results of cervical biopsy in women infected with the human immunodeficiency virus. Gynecologic Oncology 49(1): 139

Durand, L.; Tachot, J., 1972: Perforating keratoplasty associated with cataract extraction. Archives D'ophtalmologie et Revue Generale D'ophtalmologie 32(4): 269-276

Callison, A., 1992: Native Tillandsia species of Georgia. Journal of the Bromeliad Society 42(4): 172-174