+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Purification of Salmonella stanley enterotoxin and its immunology and dermatotoxicity

, : Purification of Salmonella stanley enterotoxin and its immunology and dermatotoxicity. Indian Journal of Experimental Biology 30(1): 23-25

Isolation of an enterotoxic factor from cell-free-culture-supernatant of S. stanley was achieved to homogeneity using salt precipitation, dialysis and molecular seive chromatography through Sephadex G-100 and G-200 columns. The purified enterotoxic factor yielded a single protein band on polyacrylamide gel electrophoresis, induced antibodies in the rabbit and showed single band on agar gel precipitation. It induced fluid accumulation in the rabbit ligated ileal loop (RLIL) and was neutralized by the homologous antiserum. Antigenically it was not related to cholera toxin but with enterotoxin of other Salmonella serotypes. It also exerted dermatotoxic effect in the rabbit skin causing marked central necrosis with peripheral erythema.


PMID: 1506012

Other references

Schneider, J-Christophe.; E.K.bir, D.; Chéreau, C.; Mercier, J-Christophe.; Dall'Ava-Santucci, J.; Dinh-Xuan, A.Tuan., 2002: Involvement of Na(+)/Ca(2+) exchanger in endothelial NO production and endothelium-dependent relaxation. Endothelial nitric oxide (NO) synthase (eNOS) is controlled by Ca(2+)/calmodulin and caveolin-1 in caveolae. It has been recently suggested that Na(+)/Ca(2+) exchanger (NCX), also expressed in endothelial caveolae, is involved in eNOS activation....

Barbour, A.G., 1984: Immunochemical analysis of Lyme disease spirochetes. Sera from patients with Lyme disease (LD) originating in the United States and Europe were examined by western blot analysis for antibodies to LD spirochete components. Whereas some components reacted with antibodies from the majority of patients,...

Hadjipetrou-Kourounakis, L.; Karagounis, E.; Rekka, E.; Kourounakis, P., 1989: Immunosuppression by a novel analgesic-opioid agonist. The effect of a new, centrally acting analgesic, 2-n-pentyloxy-2-phenyl-4- methyl-morpholine (PM) on the humoral antibody isotype responses, mitogenic responses, and interleukin production and assay was studied. Treatment with this opioid agonist...

Keiffer, D.; Fuhlbrigge, R.; Amerding, D.; Rossiter, H.; Kupper, T.S., 1997: Molecular identification of cutaneous lymphocyte associated antigen CLA is an isoform of P selectin glycoprotein ligand 1. Journal of Investigative Dermatology 108(4): 555

Laszlo, P.; Paris, A., 1978: Self-association of 5'-GMP: involvement of tetrameters, and inclusion of the sodium cation in the central cavity of the tetramers. Comptes Rendus Hebdomadaires des Seances de L'academie des Sciences. Serie D: Sciences Naturelles 286(9): 717-719

Lüllwitz, W., 1977: Experiences with the argon laser in prophylactic coagulation of pre-detachment conditions. Bericht Uber die Zusammenkunft. Deutsche Ophthalmologische Gesellschaft 74: 397-399

Molotkov V.N.; Mintser O.P., 1984: The role of medical cybernetics in the perfection of the diagnosis prognosis and choice of the treatment method in pulmonological patients. The use of medical cybernetics methods in pulmonology are discussed. Analysis of the results of machine and machine-free (mark) diagnosis of chronic lung diseases. Demonstrated that the quality of the disease recognition by means of the cybernetic...

de Souza, W.; Attias, M., 2016: New views of the Toxoplasma gondii parasitophorous vacuole as revealed by Helium Ion Microscopy (HIM). The Helium Ion Microscope (HIM) is a new technology that uses a highly focused helium ion beam to scan and interact with the sample, which is not coated. The images have resolution and depth of field superior to field emission scanning electron mi...

Deister, A.; Marneros, A., 1993: Long-term stability of subtypes in schizophrenic disorders: A comparison of four diagnostic systems. The long-term stability of subtypes of schizophrenic disorders in 148 narrowly defined schizophrenic patients according to four diagnostic systems was compared. The patients were investigated longitudinally for 23 years on average (range 10-50 yea...

Steinmann, H., 1960: On the cephalic nervous system of Orthopterous insects (Orthoptera). Annales Historico Naturales Musei Nationalis Hungarici, 52: 217-227