geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Quantitative analysis of immunoglobulin G subclass responses to Pseudomonas aeruginosa antigens in cystic fibrosis


, : Quantitative analysis of immunoglobulin G subclass responses to Pseudomonas aeruginosa antigens in cystic fibrosis. Journal of Medical Microbiology 36(6): 437-444

The four subclasses of IgG have different structures, functions and implications in the antibody response. IgG subclass reactions to individual Pseudomonas aeruginosa structural antigens in 22 adolescents and young adults with cystic fibrosis (CF) were studied qualitatively and quantitatively by densitometric analysis of Western blot assays. These patients had been infected by P. aeruginosa for 7 years or longer and were divided into two groups according to their pulmonary status: Group 1 comprised 11 patients with relatively good pulmonary status; Group 2 consisted of 11 patients with poor pulmonary status. There was a relative decrease of IgG1 and a relative increase of IgG2 and, especially, of IgG3 and IgG4 antibodies against P. aeruginosa antigens in the CF patients. Comparison of the two CF patients groups showed a significant increase in the proportion of IgG3 in the Group 2 patients. This could be a potential cause or effect in the deterioration of their pulmonary function. Densitometric analysis of Western blots revealed more than 24 P. aeruginosa antigens and indicated those that were the targets of the isotype antibody response(s) that were apparently most harmful. Thus, there was a significant increase of IgG2 or IgG3 reactivity (or of both) against proteins F, H (H1 and H2), and I in the Group 2 patients. One other striking observation of this study was the high reactivity of IgG4 antibodies to protein H. IgG4 was the major antibody to this protein in seven of the 11 Group 1 patients compared to two of the 11 in Group 2. We hypothesize that IgG4 antibodies may antagonise IgG2 antibodies, helping to preserve stable pulmonary function.

US$19.90

PMID: 1613784

DOI: 10.1099/00222615-36-6-437


Other references

Terrab, A.; Paun, O.; Talavera, S.; Tremetsberger, K.; Arista, M.; Stuessy, T.F., 2006: Genetic diversity and population structure in natural populations of Moroccan Atlas cedar (Cedrus atlantica; Pinaceae) determined with cpSSR markers. Atlas cedar (Cedrus atlantica) is an ecologically and economically important forest tree species of northern Africa and is considered one of the endangered conifer species in the region. Chloroplast microsatellites (cpSSR) were used to study genet...

Pearson, R.W., 1977: Acupuncture research in the USSR. Comparative Medicine East and West 5(2): 191-192

Wei, B.Y., 1983: Clinical analysis of 623 cases of intrahepatic bile duct stones. Zhonghua Wai Ke Za Zhi 21(6): 335-337

Latfullina, Rsh, 1973: Fermentative activity of yeast according to the amounts of sugar in the medium. Izvestiia Seriia biologicheskaia: 5 42-46

Corbus, B.C., 1949: Intracaine lubricant as a urethral anesthetic. Journal of Urology 62(1): 89-89

Ozawa, Y.; Nagasawa, M.; Tanigawa, N.; Tokutake, E.; Yumikura, H.; Tsuzi, M.; Watanabe, I.; Saito, T.; Saito, S.; Hatano, M., 1983: Experimental studies on the genesis of low-frequency vibrations (M-sound) of the first heart sound using a miniature accelerometer. We studied eight mongrel dogs, weighing 15 to 35 kg, in which an initial low-frequency vibration of the first heart sound (M-sound) was recognized on the chest wall. A miniature accelerometer weighing 0.5 gm was used to record surface velocity sig...

Brown, C.C.; Giddon, D.B.; Dean, E.D., 1965: Techniques Of Plethysmography. Psychophysiology 1: 253-266

Munson, M.R.; Cole, A.; Jaccard, J.; Kranke, D.; Farkas, K.; Frese, F.J., 2014: An Engagement Intervention for Young Adults with Serious Mental Health Conditions. NlmCategory="UNASSIGNED">Young adults with serious mental health conditions (SMHCs) often do not engage continuously with mental health services, and there are few engagement interventions designed for them. This qualitative study pre...

O'Grady, R.T., 1984: Changes in the somatic musculature of the 4th-stage larvae of Ascaris suum Goeze, 1782 (Nematoda: Ascaridoidea) developing in swine. Fourth-stage larvae of Ascaris suum, recovered from piglets at 11-22 days post-infection (p.i.), were examined by light microscopy for changes in the somatic musculature. During this time the estimated total number of muscle cells in the body incr...

Boyle, C.J., 2008: Congratulations on your graduation!. American Journal of Pharmaceutical Education 72(4): 94-94