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Senescence specific increase in cytosolic glutamine synthetase and its messenger rna in radish cotyledons

, : Senescence specific increase in cytosolic glutamine synthetase and its messenger rna in radish cotyledons. Plant Physiology (Rockville) 88(4): 1430-1434

Changes in the levels of cytosolic and chloroplastic isoforms of glutamine synthetase were examined in senescing radish (Raphanus sativus L. cv Comet) cotyledons by immunoblotting analysis using antibodies raised separately against maize glutamine synthetase isoforms. Translatable mRNAs for these isoforms were also examined by analyzing translation products from poly(A)+ RNA in a wheat germ system with the antibodies. The relative content of cytosolic isoform (GS1) increased twofold in the cotyledons that were placed in the dark for 72 hours to accelerate senescence, while that of chloroplastic isoform (GS2) declined to half of its initial level. The dark-treatment also increased the relative level of translatable mRNA for GS1 sevenfold after 72 hours, and decreased rapidly that for GS2 and for other nuclear-coded chloroplast proteins as well. Cotyledons also accumulated GS1 mRNA when they became senescent after a lengthy growth period under continuous light. These observations suggested that GS1 genes were activated, while those for GS2 were repressed, and eventually the population of the enzyme was altered in senescent cotyledonary cells. The role of increased cytosolic enzyme is discussed in relation to the nitrogen metabolism in senescent leaves.


Other references

Anonymous, 1971: The allergic terrain. Revue Francaise D'allergologie 11(3): 289-292

Uno, H.; Murakami, S.; Fujimoto, A.; Yamaoka, Y., 2005: Synthesis of bhimamycin B based on oxidative rearrangement of 4-acetylnaphthofuran-5-ol to naphthofuran-4,9-dione. The first total synthesis of bhimamycin B, a novel member of naphtho[2,3-c]furan quinone antibiotics, was achieved by oxidative skeletal rearrangement of 4-acetylnaphtho[1,2-b]furan-5-ol. (c) 2005 Elsevier Ltd. All rights reserved.

Kumar, S.; Kumari, R.; Sharma, V.; Sharma, V., 2014: Roles, and establishment, maintenance and erasing of the epigenetic cytosine methylation marks in plants. Heritable information in plants consists of genomic information in DNA sequence and epigenetic information superimposed on DNA sequence. The latter is in the form of cytosine methylation at CG, CHG and CHH elements (where H = A, T orC) and a varie...

Wilson S.P., 1987: Cyclic amp mediates the effects of vasoactive intestinal peptide on chromaffin cell enkephalin levels. Society for Neuroscience Abstracts 13(2): 1311

Maas, C.L.; Leek, B.F., 1985: Central and local actions of opioids upon reticulo-ruminal motility in sheep. In anaesthetized sheep, opioids depressed in an identical manner both the amplitude of spontaneous cyclical contractions and contractions evoked by electrical stimulation of the distal end of the cut cervical vagus. In conscious sheep, low doses o...

Karpova, A.I., 1936: Noxiousness of Chlorops pumilionis Bjerk. on Summer Cereals in 1935.. Data obtained from various districts in north-western and central European Russia on the degree of damage caused to summer cereals by Chlorops taeniopus, Mg. (pumilionis, Bjerk.) showed that the crop reduction is greater in barley than in wheat. I...

Scowen P.A.H.; Lavoie J.; Francis D., 1994: Implications of the compositional polarity of troctolite-anorthosite intrusions across the Grenville Province in eastern Quebec. Program with Abstracts - Geological Association of Canada(Mineralogical Association of Canada(Canadian Geophysical Union, Joint Annual Meeting 19: 101

Goundasheva, D.; Andonova, M.; Ivanov, V., 1997: Changes in lymphocyte proliferative activity in rats subjected to acute and chronic swimming stress. The changes in lymphocyte proliferative activity in a culture of whole blood were studied in rats subjected to acute and chronic stress, using 3H-thymidine. The proliferative activity of lymphocytes was found to be inhibited to a varying extent de...

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