geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Studies on cadmium toxicity in early development of mouse ova


, : Studies on cadmium toxicity in early development of mouse ova. Nichidai Igaku Zasshi 48(6): 519-532

The purpose of the present experiments was to examine the effect of cadmium on mouse germ cells when this substance was given subcutaneously to the animal in the second meiotic metaphase. Furthermore, its mechanism of action was investigated on the basis of in vitro cultivation, enzymic histochemistry and residue analysis. The progress of gestation after implantation was also followed up. The result obtained were as follows. 1) When cadmium was administered to mice at dose levels of 0.075, 0.15, 0.3, 0.6 1.2 and 2.4 mg/kg, the fertilization rate (2-cell cleavage rate) was found to be normal at 36 hours after ovulation, but the ratio of normal ova decreased at 84 hours in proportion to increase in the cadmium dose. 2) The ratio of normal ova was 59.9, 39.7 and 11.1% at 38, 62 and 86 hours after ovulation. These findings indicate that, during the process of oval growth from the 2-cell to blastocytst stage, degeneration of the ova was gradually induced. 3) To study the developmental ability of the ova as affected by cadmium, 2-cell ova obtained from cadmium-treated mice were cultured in a chemically defined medium. As a result, about 87.0% of the ova ceased to grow by the end of 48 hours of cultivation. 4) Two-cell ova obtained from mice with no cadmium treatment were cultured in a medium containing cadmium. It was found that oval growth was hampered at 4 ppm and over of the substance, but not at 2 ppm. 5) Following administration of 5 mg/kg cadmium, its content in the uterus and ovary was determined. The mean cadmium content was 1.58 .+-. 0.7 ppm for the former and 2.42 .+-. 1.4 ppm for the latter. In view of the above findings, it was clear that the oval fragility did not result from cadmium in the environment where the embryos grew up. 6) Following administration of 0.15 mg/kg cadmium, all mice were sacrificed on day 18 of gastation to examine the progress of gestation. As a result, the ratio of post- and pre-implantation loss was significantly high as compared to the control. 7) One-cell and 2-cell ova from cadmium-treated mice were examined by means of enzymic histochemistry. Although no abnormality was demonstrated in the 1-cell ove, SDH and NADH2-DH were markedly reduced in the 2-cell ova. NADH2-DH, in particular, decreased quickly from 38 post-ovulative hours onwards.

US$29.90


Other references

Dumitresco, N.; Bercea, O., 1961: The bronchial lymph-node elements in the pathogenesis of relapses in tuberculosis. La Presse Medicale 69: 1952-1954

Gupta, J.J.; Yadav, B.P.S.; Gupta, H.K., 1992: Rice bean (Vigna umbellata) as poultry feed. For 49 days 112 broiler chickens, 14 days old, were fed on diets containing 20 or 40% raw or roasted rice bean (Vigna umbellata) meal (RBM). The roasted RBM diet was supplemented with animal protein. Metabolizable energy content was higher (P<0...

Freixes J.; Parra P.; Bruseghini L.; Andreoli R., 1985: Relationship between neuronal protective activity of adafenoxate and its effect on brain enzymes. Revista de Farmacologia Clinica y Experimental 2(3): 298

Leeman, C.P., 1965: Acute Dystonic Reactions To Small Doses Of Prochlorperazine; Report Of Three Cases Misdiagnosed As Hysteria. Three patients had acute dystonic reactions to small doses of prochlorperazine (Compazine). The clinical features included trismus, dysarthria, and sensory and motor disturbances of the tongue. Because of the patients' anxiety and other facto...

Bannister S.; Melhuish A.; Henrys S.; Stern T.; ten Brink Uri, 1999: Deep seismic reflections beneath the Trans-Antarctic mountain front, from reprocessed SERIS seismic data. Terra Antartica 6(3-4): 363-364

F.Y.eGuan; Zhang FangPing; Peng ZhengQiang; Liu Kui; Jin Qian, 2002: The effects of temperature on the development and reproduction of Tetranychus piercei McGregor (Acari: Tetranychidae) in banana. Observations on the development, survival and reproduction of T. piercei reared on fresh banana leaves were conducted at 16, 20, 24, 28, 32 and 36 degrees C. The developmental time from egg to adult varied from 7.2 d at 32 degrees C to 33.1 d at 1...

Yanbin, Shen, 1994: Jurassic conchostracans from Carapace Nunatak, southern Victoria Land, Antarctica. Fossiliferous horizons of the Ferrar Group at Carapace Nunatak of southern Victoria Land have yielded the richest and most diverse freshwater Jurassic biota hitherto recorded from Antarctica. Fossil conchostracans are the most important in terms o...

Kanakry, J.A.; Ambinder, R.F., 2014: EBV-related lymphomas: new approaches to treatment. In the treatment of Epstein-Barr virus (EBV)-related lymphomas, there are few therapies specifically targeted against the latent virus within these tumors; in most cases the treatment approach is not different than the approach to EBV-negative lym...

Barbosa, F.Timbó.; da Cunha, R.Martins., 2012: Reversal of profound neuromuscular blockade with sugammadex after failure of rapid sequence endotracheal intubation: a case report. Sugammadex is a reversal agent that acts as a selective antagonist of neuromuscular blockade induced by rocuronium and vecuronium. This is a case report of an elderly female patient who had sugammadex just after rocuronium induction. An 88-year-ol...

Kennedy, C.H.; Liporace, J.; Malamut, B.L.; Sperling, M.R., 1998: Sexual dysfunction in partial epilepsy. Epilepsia 39(SUPPL 6): 178-179