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Subunit structure of porcine submaxillary mucin


, : Subunit structure of porcine submaxillary mucin. Biochemistry 28(14): 6114-6121

The structure of a high molecular weight fraction of porcine submaxillary mucin was studied by using degradative techniques. Reduction of disulfide linkages released mucin subunits together with an associated protein(s) of approximately 140 kDa. The molecular weights of the subunits ranged from approximately 0.5 .times. 106 to 2.5 .times. 106. Trypsinization of subunits generated glycosylated domains and small, poorly glycosylated or nonglycosylated tryptic peptides. The glycosylated domains, which have an average molecular weight of approximately 270K, possess an unusual amino acid composition containing only nine different amino acids. The minor amino acids which are absent from the glycosylated domains but which are consistently present in both the mucin and the mucin subunits were recovered in the tryptic peptides. Pronase digestion of the glycosylated domains generated smaller fragments of aproximately 17 kDa. Comparing these results to the partial cDNA sequence for porcine submaxillary mucin reported by Timpte et al. [(1988) J. Biol. Chem. 263, 1081-1088] suggests that the glycosylated domains consist of variable numbers of the 81 amino acid tandem repeat observed in the cDNA sequence. Further, the fact that porcine submaxillary mucin contains subunits, link proteins, and glycosylated domain suggests that its structure is simlar to that described for cervical and intestinal mucins. Intact mucin, mucin "subunits", and the glycosylated domains are all polydisperse with respect to molecular weight, indicating that mucin polydispersity is due to variability in the number of units linked together as ell as to variability in the size of the units.

US$19.90

PMID: 2775758

DOI: 10.1021/bi00440a058


Other references

Sourek, K., 1961: Sequelae in the central nervous system of resuscitation during the stage of clinical death. Rozhledy V Chirurgii 40: 698-701

Barbarick, K.A., 1985: Potassium fertilization of alfalfa grown on a soil high in potassium. The influence of up to 750 kg K/ha on semidormant Medicago sativa cv. Agate, Baker, Vernal and WL318 grown on a soil high in K (Nunn clay loam-Aridic Argiustoll) over 4 years was investigated. Fertilizer K significantly increased DM yield for the...

Janetschek, H., 1949: Tierische suc-cessionen auf hochalpinen Neuland. Nach Untersuchungen am Hintereis-, Niederjock- und Gepatschferner in den Otztaler Alpen. Bericht des Naturwissenschaftlich-Medizinischen Vereins in Innsbruck, 48-49: 1-215

Eastley, N.; Aujla, R.; Khan, Z., 2012: Radiographs late in the follow up of uncomplicated distal radius fractures: are they worth it? Clinical outcome and financial implications. Fractures of the distal radius are common. Displacement can quickly lead to secondary osteoarthritis. Early follow up radiographs are subsequently paramount to facilitate for early attempts at reduction. Developing callus eventually makes this imp...

Delagneau, J.F.; Toma, B.; Vannier, P.; Loquerie, R.; Prunet, P.; Tillon, J.P., 1975: Immunization against Aujeszky's disease with an inactivated virus vaccine in oil excipient. The vaccine was tested in rabbits and pigs. Results of serological study and challenge showed that it was more active than a commercial vaccine with saponin and Al(OH)3 adjuvant. The protection given to piglets by colostral antibodies from vaccina...

Kafes, H.; Güray, Yşim.; Gücük İpek, E., 2016: Management of cardiac device infections according to current data. The use of implantable devices in management of cardiac diseases is increasing as a result of improvements in technology of permanent pacemaker and implantable cardioverter defibrillators. Device related infections are also rising accordingly and...

Mohtadi, N.G.; Hollinshead, R.M.; Ceponis, P.J.; Chan, D.S.; Fick, G.H., 2006: A multi-centre randomized controlled trial comparing electrothermal arthroscopic capsulorrhaphy versus open inferior capsular shift for patients with shoulder instability: protocol implementation and interim performance: lessons learned from conducting a multi-centre RCT [ISRCTN68224911; NCT00251160. The shoulder is the most frequently dislocated joint in the body. Multiple causes and pathologies account for the various types of shoulder instability. Multi-directional instability (MDI) and multi-directional laxity with antero-inferior instabil...

Neprina, G.S.; Panteleeva, E.S.; Vatin, O.E.; Kariakin, O.B., 1994: The immunomodulating action of bestatin in the combined therapy of bladder cancer patients. A beneficial effect of bestatin used as adjuvant in the course of chemo- and radiotherapy of local cancer of the bladder manifested itself as a reduction of postradiation immunosuppression: increased count of T mu-helper/inductors and T gamma-supp...

Nikin V.V.; Zakharov I.Yu, 1990: Echocardiography and transesophageal electrocardiostimulation in the diagnosis of early stages of alcohol induced damage to the heart. Sovetskaya Meditsina (9): 45-48

Terasaki T.; Pardridge W.M., 1987: Differential binding of t 4 and t 3 to acidic isoforms of thyroid hormone binding globulin tbg in human sera. Clinical Research 35(3): 403A