+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Techniques for assessing canine mononuclear phagocyte function as part of an immunotoxicologic evaluation

, : Techniques for assessing canine mononuclear phagocyte function as part of an immunotoxicologic evaluation. International Journal of Immunopharmacology 10(6): 765-772

Although the dog is used extensively as a model for toxicologic investigation, few practical techniques for assessing immunotoxicity in this species have been reported. We have adapted techniques for assessing peripheral blood monocyte (PBM) function in the dog. Methods for isolating PBM were compared; a technique employing hypertonic conditioning followed by density gradient centrifugation, which resulted in > 90% purity and a greater yield of PBM than that obtained with other previously reported methods is described, along with methods for measuring antibody-dependent cellular cytotoxicity (ADCC) and the production of prostaglandin E2 (PGE2) and superoxide anion (O2-). An assay for the measurement of interleukin-1 (IL-1), based on its ability to induce interleukin-2 (IL-2) production by the EL-4 mouse thymoma line in the presence of the calcium ionophore A23187, was examined. Together, these assays provide the tools to better define drug or chemical effects on the mononuclear phagocyte system (MPS) of this important animal model.


PMID: 2848778

DOI: 10.1016/0192-0561(88)90030-6

Other references

Durante W.; Sunahara F.A., 1986: The possible involvement of prostaglandin i 2 in the positive end expiratory pressure induced changes in cardiac output and blood pressure. Ventilation with positive end-expiratory pressure (PEEP) is employed in clinical and aviation medicine. The widespread application of PEEP, however, is limited principally because of its adverse effect on cardiac output (CO) and systemic arterial...

Artre, G., 1931: Etude paleontologique. BullHist. nat. Toulouse: 345-355

Kornilov, A.A., 1949: The flowering of perilla under continuous illumination.. The work is reviewed of previous Russian workers on Perilla ocymoides, a typical short-day plant which is, stated not to flower under continuous illumination. The author presents data to show that he obtained flowering in Perilla under continuous...

Mamedov, T.A., 1965: On the Palaeogene ecology of numrnulites. Izvestiya Akademii Nauk Azerbaidzhanskoi SSR Geol Geog, 2: 3-11

Bauer, Lucas., 2004: Taxonomy and nomenclature of the genus Corallus Taxonomie en nomenclatuur van het genus Corallus. Lacerta. september-oktober; 625: 206-211

Sun Dazhong; W.C.anghua, 1981: The principal geological and geochemical characteristics of the Archaean greenstone-gneiss sequences in North China. Special Publication - Geological Society of Australia 7): 121-132

Ruckdeschel, F.R.; Walsh, A.M.; Hauser, O.G.; Stephan, C., 1978: Characterizing halftone noise: a technique. A measurement technique is presented that permits the characterization of the regularity of low-coverage halftone patterns common to many reproduction processes. The technique is based on a scanning microdensitometer, which examines individual hal...

Jung, D.W.; Bradshaw, P.C.; Litsky, M.; Pfeiffer, D.R., 2003: Ca2+ transport in mitochondria from yeast expressing recombinant aequorin. We have expressed aequorin in mitochondria of the yeast Saccharomyces cerevisiae and characterized the resulting strain with respect to mitochondrial Ca(2+) transport in vivo and in vitro. When intact cells are suspended in water containing 1.4 mM...

Mráček, Z.; Půža, V.; Nermut', J., 2016: The significance of the tail projections in female entomopathogenic nematodes within the family Steinernematidae. Several taxonomic studies of the entomopathogenic nematode family Steinernematidae have reported on the minute tail projections on the tail tips of females of the first generation; however, the occurrence and morphology of these traits in other st...

Karrer, P.; Benz, J., 1948: Axeroph-ten, der dem Vitamin A zu Grunde liegende Kohlenwasserstoff. Axerophtene, the hydrocarbon corresponding to vit. A, was synthesized, and found to be a bright yellow, oily liquid which distilled in a cathode-ray vacuum at 90 C, and showed vit. A activity. The product obtained may be a mixture of 2 iso-mers.