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The amino acid sequence of a glutamic acid rich protein from bovine retina as deduced from the complementary dna sequence

, : The amino acid sequence of a glutamic acid rich protein from bovine retina as deduced from the complementary dna sequence. Proceedings of the National Academy of Sciences of the United States of America 88(8): 3116-3119

cDNA clones encoding a glutamic acid-rich protein were isolated from a bovine retina cDNA expression library. The cDNA sequence contained an open reading frame of 1770 base pairs encoding a protein of 590 amino acids (64,509 Da) and untranslated regions of 60 and 490 base pairs at the 5' and 3' ends, respectively. The cDNA hybridized to a 2.4-kilobase retinal mRNA. The amino acid sequence derived from the cDNA sequence contains a glutamic acid-rich domain in which 68 of 109 amino acids are glutamic acid. In addition, this domain contains four repeats of a peptide of 11 amino acids and two repeats of a peptide of 26 amino acids. A polyclonal antibody raised against a decapeptide corresponding to the undecapeptide repeat sequence reacted with a protein in an extract of bovine rod outer segments, whose moelcular mass, 65 kDa, corresponded to that of the above glutamic acid-rich protein. The retinal glutamic acid-rich protein showed homology with glutamic acid-rich proteins form bovine brain and the C-terminal region of mammalian neurofilaments.


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Lemper, M.; Snykers, S.; Vanhaecke, T.; D.P.epe, K.; Rogiers, V., 2014: Current status of healthy human skin models: can histone deacetylase inhibitors potentially improve the present replacement models?. Histone deacetylase inhibitors (HDACi), a relatively new group of epigenetic agents, are being investigated as powerful chemotherapeutics because of their antiproliferative and prodifferentiation effects both in vitro and in vivo, in various tumor...

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Delwaide, J.P., 1968: On the treatment of myasthenia. Revue Medicale de Liege 23(18): 552-557

Luhan, J.A.; Lanoff, G., 1952: The tryptophan test for tuberculous meningitis: a reevaluation. A.M.A. Archives of Neurology and Psychiatry 67(6): 822-822

Thompson, E.O.; Air, G.M., 1971: Studies on marsupial proteins. VI. Evolutionary changes of beta-globins of the macropodidae and the amino acid sequence of beta-globin from Potorous tridactylus. Australian Journal of Biological Sciences 24(6): 1199-1217

Arshavsky, I.A.; Darinsky, N.V.; Li, L.A.; Siryk, L.A., 1975: Comparative ontogenetic features of skeletal muscles in physiologically mature and immature mammals. One series of experiments investigated the time required by such indices of the states of skeletal muscles as membrane potential (MP), action potential (AP), and content of total protein and dense residue to acquire values approximating those foun...

Kozma, W.A., 1953: Clinical photography focuses on teaching. Modern Hospital 80(6): 81-82