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The development of sea urchin embryos after cryopreservation


, : The development of sea urchin embryos after cryopreservation. Ontogenez 19(2): 175-180

The sea urchin embryos were cooled to -196.degree. by two-step freezing with the use of 1-1.5 M dimethyl sulfoxide as a cryoprotectant. The embryos were equilibrated with the cryoprotectant for 20-30 min at 0. .+-. 2.degree. At -7.degree. ice crystallization was induced and the embryos were cooled to -38--42.degree. at a rate of 6-8.degree./min. The embryos were then transferred into liquid nitrogen. The embryo were thawed in a water bath at 19.degree. No less than 90% of the embryos frozen at the stages of blastula, gastrula, or pluteus were capable of recovery and normal development. The length of cryopreservation did not affect the survival of the embryos.

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