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The regulation of intracellular ph estimated by phosphorus 31 nmr spectroscopy in the anterior byssus retractor muscle of mytilus edulis l


, : The regulation of intracellular ph estimated by phosphorus 31 nmr spectroscopy in the anterior byssus retractor muscle of mytilus edulis l. Journal of Experimental Biology 150: 95-110

The regulation of the intracellular pH (pHi) in the anterior byssus retractor muscle (ABRM) of Mytilus edulis L. during an acidic and alkaline load was studied by 31P-NMR spectroscopy. Under aerobic conditions, a total intracellular buffer capacity of 26.5 .+-. 0.7 mmol l-1 pH unit-1 (N = 3) was estimated. In the absence of serotonin, active acid extrusion was by a Na+-independent, primary active transport mechanism coupled to anion transport. Base equivalents appear to be extruded by a Cl-/HCO3- exchanger. Serotonin (10-5 mol l-1) induced an increase in pHi through additional activation of a Na+/H+ exchanger.

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