+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Transfructosylation catalyzed by beta fructofuranosidase i from arthrobacter sp k 1

, : Transfructosylation catalyzed by beta fructofuranosidase i from arthrobacter sp k 1. Agricultural & Biological Chemistry 54(10): 2655-2662

Transfructosylation of the .beta.-fructofuranosidase I from Arthrobacter sp. K-1 was investigated. This enzyme catalyzed both transfructosylation and hydrolytic action, when it was incubated with sucrose alone. But in the presence of a suitable acceptor such as D-xylose and lactose, the enzyme catalyzed mostly transfructosylation and transferred the fructose residue preferentially to the acceptor. The enzyme had broad acceptor specificities. D-Xylose, D-galactose, L-sorbose, D- and L-fucose, D- and L-arabinose, maltose, isomaltose, cellobiose, lactose, melibiose, xylobiose, maltotriose, methyl .beta.-glucoside, and galactoside were efficient acceptors in the transfructosylation. On the other hand, D-ribose, L-rhamnose, D-mannose, 2-deoxy-D-glucose, D-galactosamine, D-galacturonic acid, and 1-kestose were not efficient acceptors. Various primary alcohols, polyhydric alcohols including some sugar alcohols, and some glycosides acted as acceptors, but secondary alcohols with one hydroxyl group such as 2-propanol and 2-butanol were not effective as acceptors.


Other references

Sinha, S.; David, S.; Gerdin, M.; Roy, N., 2013: Vulnerabilities of Local Healthcare Providers in Complex Emergencies: Findings from the Manipur Micro-level Insurgency Database 2008-2009. Research on healthcare delivery in zones of conflict requires sustained and systematic attention. In the context of the South Asian region, there has been an absence of research on the vulnerabilities of health care workers and institutions in are...

Ng, D.K.; Schwartz, G.J.; Warady, B.A.; Furth, S.L.; Muñoz, A., 2017: Relationships of Measured Iohexol GFR and Estimated GFR With CKD-Related Biomarkers in Children and Adolescents. 2 valid and reliable estimated glomerular filtration rate (GFR) equations for the pediatric population have been developed from directly measured GFR data in the Chronic Kidney Disease in Children (CKiD) cohort: the full CKiD and bedside CKiD equa...

Downing Karen P.; Walker Roger G., 1988: Viking Formation, Joffre Field, Alberta; shoreface origin of long, narrow sand body encased in marine mudstones. AAPG Bulletin 72(10): 1212-1228

Glick, D.; Bieter, R.N.; Wright, H.N., 1950: Mucolytic enzyme systems. XIV. Effect of certain quinolines on hyaluronidase and its serum inhibitor. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 74(4): 778-780

Borowski, A.M.; Frangos, A.; McCann, J.W.; Brown, D.B., 2013: Pressure wire assessment of hemodynamic alterations after chemoembolization of hepatocellular carcinoma. The end point of chemoembolization for hepatocellular carcinoma is qualitative. We intended to determine the feasibility of measuring intra-arterial pressure changes after chemoembolization and hypothesized that pressures would increase in the dis...

Ehmann, Hans., 2006: Dragonflies in Salzburg 2000-2005 Insecta Odonata Libellenfunde im Bundesland Salzburg 2000-2005 Insecta Odonata. During the years 2000-2005, the odonate fauna in the province of Salzburg has been investigated. With a view to an exhaustive investigation of the areas in which dragonflies are found, and to create a representative basis for a "dragonfly atl...

Branham, S.E.; Habel, K., 1946: Preparation and evaluation of an irradiated toxoid from the toxin of Shigella dysenteriae. The toxin was prepd. by a modification of the method of. Farrell and Ferguson. Detoxification was carried out by 2 different irradiation technics. The 1st was by use Of a high pressure mercury vapor lamp of the Oppen-heimer-Levinson type. The 2d m...

Kovanova E.M., 1972: Thiamine metabolism in patients with athero sclerotic cardio sclerosis. Latvijas Psr Zinatnu Akademijas Vestis (1): 110-112

Boykin, L., 1976: Using diet exchanges. Nursing Care 9(9): 17-20

Vard'ja, T.; Sarapuu, L.; P., 1967: Diurnal dynamics in the content of phloridzin and flavonols in apple shoots. Phloridzin, its precursors chalcones and flavonols were determined in the leaves, bark and wood of 1-year-cld shoots of mature apple trees 7 times a day on 4 different dates during the growing season. The phloridzin Content on all sampling dates w...