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Utilization of V kappa families and V kappa exons. Implications for the available B cell repertoire


, : Utilization of V kappa families and V kappa exons. Implications for the available B cell repertoire. Journal of Immunology 147(9): 3194-3200

A molecular cloning approach was used to determine the relative utilization of 2 individual V.kappa.21 genes, 13 V.kappa. gene families, and the 4 functional J.kappa. gene segments among splenic B cells of nonimmunized BALB/c mice. Based on the observed frequency of individuals V.kappa. gene expression, we estimate that the mouse genome encodes 150 to 180 functional V.kappa. genes, and we suggest that most functional V.kappa. exons are expressed at comparable frequencies in the preimmune antibody repertoire. In contrast, clear differences in J.kappa. segment utilization were observed, J.kappa.4 being consistently underrepresented with respect to the other J.kappa. segments.

US$29.90

PMID: 1919010


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