+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A cloned CCK-A receptor transduces multiple signals in response to full and partial agonists

, : A cloned CCK-A receptor transduces multiple signals in response to full and partial agonists. American Journal of Physiology 265(5 Pt 1): G999-1004

Chinese hamster ovary (CHO) cells, stably transfected with the cloned rat CCK-A receptor, were used to study signal transduction events initiated by cholecystokinin octapeptide (CCK-8) and the partial agonist JMV-180. In single CHO-CCK-A cells loaded with fura-2, superfusion of CCK-8 (10 pM-1 nM) resulted in an increase in intracellular Ca-2+ concentration ((Ca-2+)-i). At CCK-8 concentrations lt 100 pM, the signal consisted of (Ca-2+)-i, oscillations. At higher concentrations, CCK-8 induced a typical biphasic response consisting of a large peak followed by a lower sustained plateau. Superfusion of JMV-180 also resulted in an increase in (Ca-2+)-i; in contrast to acinar cells this increase did not consist exclusively of (Ca-2+)-i oscillations. Both CCK-8 and JMV-180 increased polyphosphoinositide hydrolysis, although JMV-180 stimulated formation of only 10% as much (3H)inositol phosphates. (Ca-2+)-i signals stimulated by both CCK-8 and JMV-180 were blocked by the aminosteroid U-73122. CCK-8 (1-10 nM) increased formation of adenosine 3',5'-cyclic monophosphate (cAMP) and release of arachidonic acid in CHO-CCK cells. These increases were not mimicked by JMV-180 (10 mu-M). Furthermore, no cAMP formation or arachidonate release could be detected when cells were incubated with both JMV-180 and CCK-8. These data indicate that in CHO-CCK-A cells, unlike acinar cells, both CCK-8 and JMV-180 increase (Ca-2+)-i by similar mechanisms. However, the CCK-A receptor can differentially recognize and then activate discrete transduction pathways on binding of these two agonists.


PMID: 8238528

Other references

Chin, K.; Lee, W., 1982: Responses of soybean (Glycines max (L.) Merr) to the side-dress of calcium nitrate Louisiana. Research bulletin Southern University5, 4(1): 43-53

D'Errico, I.; Moschetta, A., 2008: Nuclear receptors, intestinal architecture and colon cancer: an intriguing link. The intestinal epithelium is structured in crypt-villus units which are responsible for its continuous renewal. These units are organized in a dynamic scenario in which proliferating progenitor cells are generated from stem cells in the crypts and...

Su, Y.; Fu, Y.; Gao, X.; Tian, Q., 2011: Discriminant learning through multiple principal angles for visual recognition. Canonical correlation has been prevalent for multiset-based pairwise subspace analysis. As an extension, discriminant canonical correlations (DCCs) have been developed for classification purpose by learning a global subspace based on Fisher discri...

Lonngren, R., 1975: Experiences of the new pharmacy system in Sweden. Journal of the American Pharmaceutical Association 15(7): 379-81, 405

Rizzi, F.; Ambroselli, V.; Bona, G.A.; Volonte, M.C.; Sala, G.; Sansone, T.; Mezzetti, M.G., 1993: Temporary respiratory prosthesis. Acta Anaesthesiologica Italica 44(2): 265-271

Baccarelli, A.; Kaufman, J.D., 2012: Ambient particulate air pollution, environmental tobacco smoking, and childhood asthma: interactions and biological mechanisms. American Journal of Respiratory and Critical Care Medicine 184(12): 1325-1327

Anonymous, 2011: The Moving Eye is Easy to Spy How Motion Improves Gaze Discrimination. Research indicates that people are remarkably good at discriminating where another person is looking (Gibson Bock, Dicke, Olk, Symons, & Kingstone, 28). This theory is based on research that has used static images of eye direction. In real life, h...

Kumar, K.; Kaur, H.; Deshmukh, R., 2017: Neuroprotective role of GABA B receptor modulation against streptozotocin-induced behavioral and biochemical abnormalities in rats. Stimulation as well as inhibition of GABA<sub>B</sub> (Gamma amino butyric acid) receptors has been reported to show beneficial effects in Alzheimer's disease (AD). Experimental evidences suggest that the use of GABAergic agents c...

Lemtiri-Chlieh, F.; Macrobbie, E.A.C., 1994: Role of calcium in the modulation of Vicia Guard cell potassium channels by abscisic acid: A patch-clamp study. There is evidence for a role of increased cytoplasmic Ca-2+ in the stomatal closure induced by abscisic acid (ABA), but two points of controversy remain the subject of vigorous debate-the university of Ca-2+ as a component of the signaling chain,...

Ozturk, H.S.; Durak, I.; Kavutcu, M.; Kacmaz, M.; Avcy, A.; Horasanly, E.; Cimen, M.Y.B., 2000: Effects of isoflurane on nitric oxide metabolism and oxidant status of rat myocardium. Biochemical Society Transactions 28(5): A203, October