geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A high performance liquid chromatographic method for the separation of hexose monophosphates and UDP glucose from plant extracts and its use to determine specific activities in radiotracer experiments


, : A high performance liquid chromatographic method for the separation of hexose monophosphates and UDP glucose from plant extracts and its use to determine specific activities in radiotracer experiments. Phytochemical Analysis 5(1): 10-14

Anion exchange high performance liquid chromatography (HPLC) was used to separate UDP glucose (UDPGlc) and hexose monophosphates (HMPs) in crude ethanol extracts of potato tubers. Detection and quantification of UDPGlc and (U-14C)UDPGlc was achieved with UV (A-254) and radioactivity monitors connected in series. The 14C content in glucose 6-phosphate (Glc-6-P), fructose 6-phosphate (Fru-6-P) and glucose 1-phosphate (Glc-1-P) was obtained following their enzymatic conversion to gluconate 6-P and subsequent separation by HPLC. Individual HMPs were quantified by enzyme-coupled reaction systems using a microplate reader equipped with a 340 nm filter. This approach allowed the specific activities of UDPGlc, Glc-6-P, Fru-6-P and Glc-1-P to be quantified in radiolabeled potato extracts within 2 h.

US$19.90

DOI: 10.1002/pca.2800050104


Other references

Wolfaardt, G.M.; Korber, D.R.; Lawrence, J.R., 2007: Cultivation of microbial consortia and communities. This chapter describes the tradition in environmental microbiology of addressing Koch's postulates for ascribing function or effect of to a single species and for the use of enrichment culturing to ultimately isolate guilds performing the sam...

Dubois, M., 1964: Contribution To The Histophysiology Of The Epididymis In The Bull. Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales 158: 1490-1493

Valdes, A.; Leal, D.; Vasquez, C., 1996: Alternatives for a simultaneous heating of the mixed juice and cooling of the condensate for imbibition. Possibilities were studied for using the various condensate streams from the evaporation station in order to give a required imbibition water temperature (70 or 85 degrees C) and to maximize the rise in mixed juice temperature. Comparative data ar...

McClintock, S.; Thomson, P.C.; McClintock, A.E.; Raadsma, H.W.; Tier, B., 2006: Simulating past selection for molecular reeding program evaluation. We simulated a genotype that reflected the observed phenotype, and that complied with Mendel's first law. Another important goal was to maximize the flexibility of our simulation so that a variety of traits, populations and underlying models...

Berkley, J.A., 2014: Randomised trials in developing countries. Archives of Disease in Childhood 99(7): 607-608

Kobayashi, H.; Koyama, Y.; Barrett, T.; Hama, Y.; Regino, C.A.S.; Shin, I.Soo.; Jang, B-Su.; Le, N.; Paik, C.H.; Choyke, P.L.; Urano, Y., 2009: Multimodal nanoprobes for radionuclide and five-color near-infrared optical lymphatic imaging. Current contrast agents generally have one function and can only be imaged in monochrome; therefore, the majority of imaging methods can only impart uniparametric information. A single nanoparticle has the potential to be loaded with multiple payl...

Grossi-Paoletti, E.; Paoletti, P.; Schiffer, D.; Fabiani, A., 1969: Experimental rat tumors induced with administration of nitrosourea derivatives. II. Observations on the sterol composition. Acta Neurologica 24(4): 563-564

Villadsen, J.H.; Langkjer, S.T.; Ebbesen, P.; Bjerring, P., 1987: Syngrafting skin among mice of similar and different ages increases the number of Langerhans cells and decreases responsiveness to 1,4-dinitrofluorobenzene. Counting on suction blister skin biopsies, the number of Langerhans cells per mm2 in BALB/c mouse epidermis was found identical in 1-, 11-, and 22-month-old animals, but consistently elevated in syngrafted skin 10 months after transplantation. Thi...

Van Heerden C., 1968: Prolonged obstructive jaundice in bantu infants enz trans aminase enz alkaline phosphatase. South African Medical Journal: 518-526

Smirnov S.V., 1997: Pedomorphic development of the modern amphibians. Priroda 1997(2): 77-83