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A human RNA polymerase II complex containing factors that modify chromatin structure

, : A human RNA polymerase II complex containing factors that modify chromatin structure. Molecular and Cellular Biology 18(9): 5355-5363

We have isolated a human RNA polymerase II complex that contains chromatin structure remodeling activity and histone acetyltransferase activity. This complex contains the Srb proteins, the Swi-Snf complex, and the histone acetyltransferases CBP and PCAF in addition to RNA polymerase II. Notably, the general transcription factors are absent from this complex. The complex was purified by two different methods: conventional chromatography and affinity chromatography using antibodies directed against CDK8, the human homolog of the yeast Srb10 protein. Protein interaction studies demonstrate a direct interaction between RNA polymerase II and the histone acetyltransferases p300 and PCAF. Importantly, p300 interacts specifically with the nonphosphorylated, initiation-competent form of RNA polymerase II. In contrast, PCAF interacts with the elongation-competent, phosphorylated form of RNA polymerase II.


PMID: 9710619

DOI: 10.1128/mcb.18.9.5355

Other references

Tatar, J., 1974: Sensitivity of tubercle bacilli to pyrazinamide determined on the basis of their sensitivity to nicotinamide and their pyrazinamidase and nictinamidase activities. Gruzlica i Choroby Pluc; Tuberculosis et Pneumonologia 42(8): 773-777

Lahiri, D.C.; More, V.Y., 1952: Naturally Developed Diphtheria Antitoxin Titre of a Random Indian Population. Indian Medical Gazette 87(10): 448-449

Konoki, K.; Sugiyama, N.; Murata, M.; Tachibana, K.; Hatanaka, Y., 2000: Development of biotin-avidin technology to investigate okadaic acid-promoted cell signaling pathway. Four biotin conjugates of okadaic acid were synthesized for evaluating their interactions with protein phosphatase 2A (PP2A) by surface plasmon resonance (SPR). C7-biotinylated okadaic acid exhibited the strongest binding affinity to the enzyme, w...

Shchukin S.I., 1970: The Bog fault in southern Hissar. Four stages of formation, lower Carboniferous, middle-upper Carboniferous, Permian, and Tertiary-Quaternary, structural controls of Carboniferous volcanic and plutonic complexes, and Hercynian hydrothermal formations, USSR.

Zhang, S.; Ding, F.; Luo, A.; Chen, A.; Yu, Z.; Ren, S.; Liu, Z.; Zhang, L., 2007: XIAP is highly expressed in esophageal cancer and its downregulation by RNAi sensitizes esophageal carcinoma cell lines to chemotherapeutics. X-linked inhibitor of apoptosis protein (XIAP) is the most potent member of the IAP gene family in terms of its ability to inhibit caspases and suppress apoptosis. In this study, we investigated the expression of XIAP in esophageal cancer tissues...

Davenport, A., 2004: Anticoagulation for continuous renal replacement therapy. Contributions to Nephrology 144: 228-238

Vasil yev Y.I.; Ivanova L.A.; Fomichev V.B.; Shteynberg V.V.; Shcherbo M.N., 1972: The measurement of deformations of the ground in an earthquake. Displacement velocities and deformation measured at Druzhba, USSR, October-December, 1970, English translation of Russian article.

Ossowska, G.; Klenk-Majewska, B.; Zebrowska-Lupina, I., 1996: Acute effect of dopamine agonists and some antidepressants in stress-induced deficit of fighting behavior. The effect of dopamine (DA) agonists (apomorphine, quinpirole) and three antidepressants (selegiline, nomifensine, imipramine), given in a single dose, on the electric footshock-induced fighting behavior was investigated in the control and chronic...

Stanley Clifford R.; Smee Barry W., 2007: Strategies for reducing sampling errors in exploration and resource definition drilling programmes for gold deposits. Sampling error is the degree to which the concentration of an element differs from the true element concentration of the material from which the sample was collected. Gold mineralization commonly exhibits sampling errors as large as 50-100%. As a...

Ookawa T.; Shoumura K., 1973: Effect of intra venously administered strychnine on the electro encephalogram recorded from the deep structure of the adult chicken telencephalon. Poultry Science 52(2): 806-808