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A new method for determining anti-B cell antibodies and their specificity using flow cytometry

, : A new method for determining anti-B cell antibodies and their specificity using flow cytometry. Journal Of Immunological Methods. 164(1): 21-25

We describe a new flow cytometric B cell crossmatch method with improved sensitivity and specificity. It is based on the coating of B cell surface immunoglobulins with an unconjugated polyvalent anti-human immunoglobulin antibody. The method also provides a means for determining the specificity of anti-B cell antibodies and, potentially, for anti-HLA class II antibody specificity differentiation (DR, DQ or DP) in a binding inhibition test using mouse monoclonal antibodies directed against human HLA class II antigens.


PMID: 8360506

DOI: 10.1016/0022-1759(93)90271-8

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