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A new method for determining anti-B cell antibodies and their specificity using flow cytometry


, : A new method for determining anti-B cell antibodies and their specificity using flow cytometry. Journal Of Immunological Methods. 164(1): 21-25

We describe a new flow cytometric B cell crossmatch method with improved sensitivity and specificity. It is based on the coating of B cell surface immunoglobulins with an unconjugated polyvalent anti-human immunoglobulin antibody. The method also provides a means for determining the specificity of anti-B cell antibodies and, potentially, for anti-HLA class II antibody specificity differentiation (DR, DQ or DP) in a binding inhibition test using mouse monoclonal antibodies directed against human HLA class II antigens.

US$19.90

PMID: 8360506

DOI: 10.1016/0022-1759(93)90271-8


Other references

Fournel, J.; Celice, J.; Hillion, P., 1952: Therapy of experimental parathion intoxication. Archives des Maladies Professionnelles de Medecine du Travail et de Securite Sociale 13(2): 160-168

Israelstam, Gf, 1983: Uptake of inorganic phosphate in sweet potato discs aged in the presence or absence of gibberellic acid Ipomoea batatas. Zeitschrift fur Pflanzenphysiologie = International journal of plant physiology09(1): 7-11

Osborne, D.; Wall, T., 1984: Carrion crow nest constructed largely of man-made materials. Birds in Greater Manchester, 44 No. 8

Do, T.P.T.; Leung, M.; Fuss, M.; Garcia, G.; Blanco, F.; Ratnavelu, K.; Brunger, M.J., 2011: Excitation of electronic states in tetrahydrofuran by electron impact. We report on differential and integral cross section measurements for the electron impact excitation of the three lowest lying Rydberg bands of electronic states in tetrahydrofuran. The energy range of the present experiments was 15-50 eV with the...

Dykyi I.L.; Kholup'yak I.Yu; Shevel'ova N.Yu; Sylayeva L.F.; Volkova N.O.; Filipchenko S.M., 1991: Methodology of microbiological investigations used in new preparation and drug form development. Farmatsevtychnyi Zhurnal (Kiev) (4): 37-39

Mel'nik, V.M., 1979: Endorectal "Shchetkin symptom" in acute surgical diseases and injuries of the abdominal organs. The results of the digital rectal examination of 218 patients with "acute abdomen" syndrome are generalized here. 145 patients (66.5%) showed a greater pain-fullness at the detachment of the finger from the anterior wall of the rectum th...

Clarkson B.D., 1974: The survival value of the dormant state in neoplastic and normal cell populations. Clarkson, Bayard And Renato Baserga (Ed ) Cold Spring Harbor Conferences on Cell Proliferation, Vol 1 Control Of Proliferation in Animal Cells Xiii+1029p Illus Cold Spring Harbor Laboratory: Po Box 100, Cold Spring Harbor, N Y 11724, U S A 945-972

Murray, P.R., 1994: Antimicrobial susceptibility tests: testing methods and interpretive problems. Advances in Experimental Medicine and Biology 349: 15-25

Grahn, A.; Hemdal, B.; Andersson, I.; Ruschin, M.; Thilander-Klang, A.; Börjesson, S.; Tingberg, A.; Mattsson, S.; Håkansson, M.; Båth, M.; Månsson, L.G.; Medin, J.; Wanninger, F.; Panzer, W., 2005: Clinical evaluation of a new set of image quality criteria for mammography. The European Commission (EC) quality criteria for screen-film mammography are used as a tool to assess image quality. A new set of criteria was developed and initially tested in a previous study. In the present study, these criteria are further ev...

Gavrilov, S.G., 1960: Experience with the use of intratracheal anesthesia in association with muscle relaxants in surgery. Trudy Leningradskogo Sanitarno-Gigienicheskogo Meditsinskogo Instituta 59(Suppl): 12-21