geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A novel approach for comparative study of periosteum, muscle, subcutis, and skin microcirculation by intravital fluorescence microscopy


, : A novel approach for comparative study of periosteum, muscle, subcutis, and skin microcirculation by intravital fluorescence microscopy. Microvascular Research 56(1): 30-42

Herein, we report a new model, which allows comparative study of the microcirculation of different peripheral tissues, i.e., periosteum, skeletal muscle, subcutis, and skin. Using dextran-insensitive Wistar rats gracilis and semitendinosus muscles of the left hindlimb were prepared in association with their appertaining tibial fragments, subcutis, and skin. Blood supply was guaranteed by the femoral artery via the saphenous vessels. High-resolution intravital epi-illumination microscopy of the two muscles displayed the typical microvascular architecture with the capillaries running in parallel to each other (capillary density (CD) 128.4 +/- 4.5 cm-1). In subcutis and skin, capillaries were found arranged as interconnecting mesh-like networks with a density, which was significantly higher (P < 0.05) in subcutis (191.0 +/- 5.5 cm-1) compared with skin (108.9 +/- 3.3 cm-1). Analysis of periosteal tissue revealed two distinct types of arrangements of microvascular architecture. Adjacent to the major feeding and draining vessels of the periosteum, capillaries were organized in densely meshed shunt-like networks, revealing the highest capillary density (242.7 +/- 13.2 cm-1; P < 0.05) of all tissues studied. Periosteal capillaries distant from the major feeding and draining vessels were arranged in parallel to the longitudinal axis of the tibial bone and presented with a density similar to that of the skeletal muscle (128. 6 +/- 9.4 cm-1). Topical application of acetylcholine for analysis of physiological reactivity of the microvasculature showed dose-dependent arteriolar dilation. Moreover, a 3-min upstream femoral artery occlusion demonstrated an appropriate hyperemic response in all tissues studied, indicating intact myogenic control. A prolonged period of ischemia (120 min) followed by reperfusion (60 min) caused massive (P < 0.05) leukocyte-endothelial cell interaction in postcapillary venules, similarly as reported in other microvascular tissue preparations. We propose that the model presented provides a good approach to all peripheral tissues for both the analysis of the physiology of tissue-confined microvascular control and the development of novel therapeutic strategies to counteract manifestation of nutritional dysfunction and inflammatory response in disease.

US$19.90

PMID: 9683561

DOI: 10.1006/mvre.1998.2077


Other references

Albert, P., S.; Mcfarland, H., F.; Smith, M., E.; Frank, J., A., 1994: Time series for modelling counts from a relapsing-remitting disease: Application to modelling disease activity in multiple sclerosis. Many chronic diseases are relapsing-remitting diseases, in which subjects alternate between periods with increasing and decreasing disease activity; relapsing-remitting multiple sclerosis is an example. This paper proposes two classes of models fo...

Dündar, H.; Ozgül, Rıza.Köksal.; Yalnızoğlu, D.; Erdem, S.; Oğuz, K.Karlı.; Tuncel, D.; Temuçin, Cğrı.Mesut.; Dursun, A., 2012: Identification of a novel Twinkle mutation in a family with infantile onset spinocerebellar ataxia by whole exome sequencing. Whole exome sequencing combined with homozygosity mapping comprises a genetic diagnostic tool to identify genetic defects in families with multiple affected members, compatible with presumed autosomal recessively inherited neurometabolic/neurogene...

Windham, C.T.; Helm, A.A.; Wyse, B.W., 1990: Integrity of small data bases in computer analysis of dietary data. The integrity of data bases to support microcomputer-based dietary analysis programs has become increasingly important to developers and users of nutritional analysis software. This paper reviews critical issues in maintaining data integrity durin...

Bronsteen, R.; Goyert, G.; Bottoms, S., 1989: Classification of twins and neonatal morbidity. Prematurity is the most significant determinant of neonatal morbidity among twins, but the relative importance of other factors is unclear. We compared the prognostic value of intrauterine growth retardation, discordancy, and other classifications...

Barreiro, A.; Fdez de Troconiz, P.; Vila, M.; Lopez Beceiro, A.M.; Pereira, J.L., 2003: Congenital skeletal abnormalities in a tawny owl chick (Strix aluco). In this case report, we describe a tawny owl chick (Strix aluco) coming from a Wild Fauna Recovery Center with multiple congenital malformations in the limbs. The animal was unable to fly and showed marked malnutrition and poor general appearance....

Yang, J-Ming.; Hildebrandt, B.; Luderschmidt, C.; Pollard, K.Michael., 2003: Human scleroderma sera contain autoantibodies to protein components specific to the U3 small nucleolar RNP complex. To determine whether antifibrillarin autoantibodies in scleroderma patients are associated with autoantibodies to protein components specific for U3 small nucleolar RNP (U3 snoRNP). Sera from 220 scleroderma patients were examined for antinucleola...

Rumyantsev G.I.; Khodykina T.M.; Arkhangel'skii V.N.; Nesterenko L.N., 1991: On the labor conditions and health related measures in waste materials processing of phosphorus containing fertilizer production. In the processing of the waste products of phosphorus containing fertilizer production, several major health-related factors were identified depending on the industrial site's peculiarities: unfavourable microclimate conditions (both cold and...

Colognesi, C.; Halapi, E.; Jansson, M.; Hodara, V.; Steuer, G.; Tresoldi, E.; Leitner, T.; Scarlatti, G., 1996: The role of virologic and immunologic factors in mother-to-child transmission of HIV-1. This study suggests that transmission is governed by an interaction of both viral and immunological factors. The results obtained indicate that different strategies can be applied for the prevention of transmission.Editorial comment:Most HIV infec...

Axe, J.; Zeimelis, M.R.; Zink, M.R., 1980: Turning your patients - mechanically. Rn 43(9): 47-49

Konieczny, Wacaw, 1963: Wp yw preparatow nowokainowych na przemiane azotowa u starych szczurow The effect of Novocaine on the nitrogen metabolism in old rats. Two series of studies were performed for 48 and 58 days respectively. Total N was estimated in urine with the Kjeldhal micromethod. The rats were given 0,1 or X 0,2 cc of the preparation 3 times a week. The following preparations were studied: Ger...