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ATP-MgCl2 restores depressed endothelial cell function after hemorrhagic shock and resuscitation


, : ATP-MgCl2 restores depressed endothelial cell function after hemorrhagic shock and resuscitation. American Journal of Physiology 268(4 Pt 2): H1390-6

Although ATP-MgCl-2 produces beneficial effects following various adverse circulatory conditions, it remains unknown whether this agent restores the depressed endothelial cell function (i.e., the reduced release of endothelium-derived nitric oxide (EDNO) and endothelium-derived contracting factors (EDCF)) in a model of trauma-hemorrhage and resuscitation. To determine this, rats underwent laparotomy (i.e., trauma induced), were bled to and maintained at a mean arterial pressure of 40 mmHg until 40% of shed blood volume was returned in the form of Ringer lactate (RL). The animals were then resuscitated with four times the volume of maximal bleedout with RL, following which ATP-MgCl-2 (50 mu-mol/kg body wt) or saline was administered. At 1.5 h postresuscitation, the aorta and superior mesenteric artery (SMA) were isolated, and dose-responses for acetylcholine (ACh, an endothelium-dependent vasodilator, via EDNO) and nitroglycerin (an endothelium-independent vasodilator) were determined. In addition, hypoxia-induced contraction, a process mediated by EDCF, was assessed. The results indicate that the decreased endothelium-dependent relaxation after hemorrhage (sham 94 +- 3 and 97 +- 3% vs. hemorrhage 64 +- 5 and 57 +- 11% at 10-5 M ACh in aorta and SMA, respectively, P lt 0.05) was restored with ATP-MgCl-2 treatment. In contrast, there was no significant difference in nitroglycerin-induced relaxation. Moreover, the decreased hypoxia-induced aortic contraction after hemorrhage (sham 221 +- 26 mg/ring vs. hemorrhage 124 +- 22 mg/ring, P lt 0.05) was attenuated by administration of ATP-MgCl-2. Because ATP-MgCl-2 restores the depressed endothelial cell function, this agent appears to be a useful adjunct for protecting the endothelium after trauma and hemorrhage, even in the absence of blood resuscitation.

US$29.90

PMID: 7733338


Other references

Kazuno, K.; Ohtani, N.; Nakanishi, S., 2012: Two cases of endovascular abdominal aortic aneurysm repair with iliac aneurysm using a zenith iliac bifurcation graft. We report on treatment of an abdominal aortic aneurysm with common iliac artery aneurysm using an iliac branch device. We performed 2 cases because of a large common iliac artery aneurysm or a complication of an internal iliac artery aneurysm. Bot...

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Tucker, W.B.; Adams, G.D.; Lema, M.; Aslam, M.; Shin, I.S.; L.R.yet, P.; Weeks, D.L., 1992: Evaluation of a system for rating edema in dairy cattle. Our objective was to evaluate a subjective edema rating system to quantitate edema severity. Edema was evaluated with 20 nulliparous Holstein heifers from 3 wk before their expected calving date until 3 wk postpartum. Severity of udder edema was e...

Nikol'skii N.N.; Bogdanova N.P.; Vinogradova N.A.; Nesterov A.M.; Sorkin A.D.; Sorokin A.B., 1966: Stimulatory action of the epidermal growth factor and insulin on urine phosphorylation in 3t3 and 3t6 cells. The influence of epidermal growth factor (EGF) and insulin on uridine phosphorylation was investigated in cell cultures Swiss 3T3 and 3T6, arrested in the medium with serum content of 0.5%. It is shown that following 5-10 minutes the addition of E...

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