geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Additivity of protein-guanine interactions in ribonuclease T1


, : Additivity of protein-guanine interactions in ribonuclease T1. Journal of Biological Chemistry 272(15): 9635-9639

It has been established that Tyr-42, Tyr-45, and Glu-46 take part in a structural motif that renders guanine specificity to ribonuclease T1. We report on the impact of Tyr-42, Tyr-45, and Glu-46 substitutions on the guanine specificity of RNase T1. The Y42A and E46A mutations profoundly affect substrate binding. No such effect is observed for Y45A RNase T1. From the kinetics of the Y42A/Y45A and Y42A/E46A double mutants, we conclude that these pairs of residues contribute to guanine specificity in a mutually independent way. From our results, it appears that the energetic contribution of aromatic face-to-face stacking interactions may be significant if polycyclic molecules, such as guanine, are involved.

US$19.90

PMID: 9092491

DOI: 10.1074/jbc.272.15.9635


Other references

Phillips, K.D., 1996: Regulating complementary medicine. Statutory regulation is essential. Bmj 313(7061): 882-882

Malmer, A.; Johansson, E.; Kluge, M., 1998: Natural rehabilitation of disturbed tropical rainforest soils in Sabah, Malaysia. The natural rehabilitation of forest soils in the title region disturbed by timber extraction was quantified by studying the dry bulk density, organic matter content, and infiltration capacity in crawler tractor tracks of different ages. The influ...

Phipps, J.B., 1996: Reduction of skin irritation during electrotransport. Official Gazette of the United States Patent & Trademark Office Patents 1188(2): 1095-1096, July 9

Welch, R.A.; Pellett, S., 1988: Transcriptional organization of the Escherichia coli hemolysin genes. The transcriptional organization of the Escherichia coli hemolysin genes (hlyCABD) encoded by pSF4000 was examined. The use of different hemolysin gene-specific radiolabeled probes in blots containing isolated in vivo RNA revealed 4.0-kilobase hly...

Mckenna J.E.; Melzack R., 1990: Analgesia produced by lidocaine injection into rat dentate gyrus. Society for Neuroscience Abstracts 16(1): 563

Zakhariev, B., 1963: On The Treatment Of Psoriasis. Vestnik Dermatologii i Venerologii 37: 76-77

Catić, D.; Lovrincević, A., 1985: Correlation of computed tomography and conventional hypopharyngography findings in hypopharyngeal tumours. Accuracy and clinical application of computed tomography (CT) and conventional hypopharyngography have been evaluated in 32 patients with hypopharyngeal carcinoma. Radiologic results were compared with endoscopic findings. Equivalent CT and hypoph...

Mignon, M.; Lewin, J.M.; Vatier, J., 1992: Pharmacology of gastric antisecretory agents and of modifying drugs of the digestive motility. Current methodological developments; introductory discussion. Therapie 47(2): 91-92

Ducret, V.; Gonzalez, M.R.; Scrignari, T.; Perron, K., 2016: OprD Repression upon Metal Treatment Requires the RNA Chaperone Hfq in Pseudomonas aeruginosa. NlmCategory="UNASSIGNED">The metal-specific CzcRS two-component system in Pseudomonas aeruginosa is involved in the repression of the OprD porin, causing in turn carbapenem antibiotic resistance in the presence of high zinc concentrat...

Ngabé, Bé.; Poissant, L., 2003: Polycyclic aromatic hydrocarbons in the air in the St. Lawrence Basin (Québec). High-volume air samples were collected from 1993 to 1996 in rural areas of Québec to investigate on the levels and the vapor-particle partitioning of polycyclic aromatic hydrocarbons (PAHs). Ranges for the mean concentrations of total PAHs (ng m(...