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Adenosine transport inhibition ameliorates postischemia hypoperfusion in pigs


, : Adenosine transport inhibition ameliorates postischemia hypoperfusion in pigs. Brain Research 734(1-2): 261-268

Cerebral ischemia is often followed by a period of delayed hypoperfusion that may contribute to tissue injury. We tested the hypothesis that augmentation of interstitial adenosine can improve tissue perfusion under this condition. 10 min global ischemia was produced in two groups of isoflurane-anesthetized newborn pigs by occlusion of subclavian and brachiocephalic arteries, and changes in local cortical blood flow and cortical interstitial purine metabolites were measured using the combined hydrogen clearance-microdialysis technique. In one group, the dialysis probe was perfused with artificial cerebrospinal fluid buffer containing nitrobenzyl-thioinosine (NBTI, 100 mu-mol/l), a competitive inhibitor of adenosine transport. In the untreated group (n = 9), baseline cortical blood flow (39 +- 3 ml/min/100 g) was depressed by 51 +- 5% and 42 +- 6% at 40 and 60 min, respectively, of postischemic reperfusion. NBTI increased baseline interstitial adenosine levels 2.4-fold which increased baseline cortical blood flow 1.5-fold to 60 +- 4 ml/min/100 g, and increased both absolute adenosine levels as well as adenosine as a percentage of total purine metabolites throughout ischemia and reperfusion. As a result, the extent of postischemic hypoperfusion was significantly lessened in NBTI-treated animals (n = 9), with reductions in cortical blood flow of only 28 +- 3% and 24 +- 5% at 40 and 60 min of reperfusion, respectively. These results indicate that inhibition of adenosine transport by NBTI elevates interstitial adenosine concentration during and following cerebral ischemia, and concomitantly improves cortical perfusion in the post-ischemic period. The latter effect may contribute to the documented neuroprotective efficacy of adenosinergic therapy in cerebral ischemia.

US$29.90

PMID: 8896833


Other references

Krzakowa, M.; Drapikowska, M., 2000: Sexual reproduction of Phragmites australis (Poaceae) in Baczkowski Pond (Poznan, Poland) revealed by peroxidase polymorphism. Four populations of Phragmites australis (Cav.) Trin. ex Steud. growing within the Baczkowski Pond (Poznan, Poland) were examined in respect to 8 morphological traits of the panicle and three enzymatic loci of peroxidase in order to determine diff...

Odum, C.U.; Isika, A.N.; Lambo, A.O., 1993: Induction of labour with single insertion of vaginal tablet of prostagladin E2 (PGE2), amniotomy, and oxytocin infusion. A randomised controlled study of the clinical value of single insertion of vaginal tablets of prostagladin E2 (PGE2) containing 3mg of dinoprostin for induction of labour in patients with Low Bishop Scores (B/S less than 5) was carried out on 20 s...

Rice E.W.; Temel S., 1981: Food and drug administration proposed regulations on clinical chemistry clinical toxicology devices. Clinical Chemistry 27(6): 1060

Deo, D.D.; Rao, A.P.; Bose, S.S.; Ouhtit, A.; Baliga, S.B.; Rao, S.A.; Trock, B.J.; Thouta, R.; Raj, M.H.G.; Rao, P.N., 2008: Differential effects of leptin on the invasive potential of androgen-dependent and -independent prostate carcinoma cells. Obesity has been linked with an increased risk of prostate cancer. The formation of toxic free oxygen radicals has been implicated in obesity mediated disease processes. Leptin is one of the major cytokines produced by adipocytes and controls bod...

Bonfiglio, M.; Jeter, W.S., 1972: Immunological responses to bone. Clinical Orthopaedics and Related Research 87: 19-27

Umapathi, R.; Reddy, P.Madhusudhana.; Kumar, A.; Venkatesu, P.; Chang, C-Jung., 2016: The biological stimuli for governing the phase transition temperature of the "smart" polymer PNIPAM in water. A lack of sufficient knowledge regarding the behaviour of stimuli-responsive polymers to biological stimuli hinders the potential use of responsive polymers as biomaterials and medical devices. Hence, in this study, we demonstrate the impact of va...

Arnoult, M.D., 1952: Localization of sound during rotation of the visual environment. American Journal of Psychology 65(1): 48-58

Diehl, W.E.; Johnson, W.E.; Hunter, E., 2013: Elevated rate of fixation of endogenous retroviral elements in Haplorhini TRIM5 and TRIM22 genomic sequences: impact on transcriptional regulation. All genes in the TRIM6/TRIM34/TRIM5/TRIM22 locus are type I interferon inducible, with TRIM5 and TRIM22 possessing antiviral properties. Evolutionary studies involving the TRIM6/34/5/22 locus have predominantly focused on the coding sequence of th...

Lange P.; Steiner W., 1984: Scanning electron microscopic investigations of calcified wood remains from the Pleistocene travertine of Weimar. Quartaerpalaeontologie 5: 225-236

Enoch, J.M., 1964: Validation Of An Indicator Of Mammalian Retinal Receptor Response: Action Spectrum. Journal of the Optical Society of America 54: 368-374