+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Alendronate modulates osteogenesis of human osteoblastic cells in vitro

, : Alendronate modulates osteogenesis of human osteoblastic cells in vitro. Japanese Journal of Pharmacology 66(1): 25-33

The bisphosphonates, which are carbon-substituted pyrophosphates, have been studied extensively both in vivo and in vitro to elucidate their effects on bone tissues and cells. However, because these agents were shown to have a potent inhibitory effect on bone resorption, the majority of studies have focused on only this aspect of bone metabolism. There appears to be less information regarding the direct effect of bisphosphonates on bone formation, so thus we undertook experiments to investigate the effects of bisphosphonates, especially alendronate, on the mineralization and matrix protein synthesis of human osteoblastic cells in vitro. The data show that the bisphosphonates, alendronate, etidronate and pamidronate, suppressed 1,25-dihydroxycholecalciferol (1,25(OH)-2D-3)-stimulated mineralization of human osteoblastic cells at high concentrations, while relatively lower concentrations of alendronate and etidronate potentiated mineralization of the cells in the presence of 1,25(OH)-2D-3. The potentiation of mineralization with alendronate was accompanied by increased synthesis of bone matrix proteins, osteocalcin and collagen, and the mRNA of pro alpha(I) collagen. These findings show that in addition to their well-known effects on bone resorption, bisphosphonates have significant and direct effects on osteogenesis in osteoblasts in vitro. The actual mechanism remains to be further investigated.


PMID: 7861665

DOI: 10.1254/jjp.66.25

Other references

Anonymous, 1990: The nuttiest peanut butter. Thirty-seven peanut butter products were rated for taste, price, and sodium content. The nutrient content and aflatoxin contamination of peanut butter were discussed.

Al-Mazroua, H.A.; Al-Rasheed, N.M.; Korashy, H.M., 2013: Downregulation of the cardiotrophin-1 gene expression by valsartan and spironolactone in hypertrophied heart rats in vivo and rat cardiomyocyte H9c2 cell line in vitro: a novel mechanism of cardioprotection. The incidence, prevalence, and hospitalization rates associated with heart failure (HF) are projected to increase substantially in the world. Among all medications used clinically to treat HF, valsartan (VAL) and spironolactone (SPL) have been sho...

Huang J.W.; Sun S.K.; Juang C.F., 1986: Studies on the integrated control of radish yellows caused by fusarium oxysporum f sp raphani. Radish yellows, caused by Fusarium oxysporum f. sp. raphani, was first found at Hsin-she county of Taichung prefecture in 1981. Meanwhile, it was also found in other radish growing areas in Hsinchu and Nantou prefectures. The field soils which had...

Fulleborn, F., 1931: Klinisches und biologisches uber Ascaris lumbricoides. Internatl. Fortbildungskursus Balneologie und Balneotherapie Jena: 38 pp.

Bertoin P.; Cros P., 1972: Condylar metastasis of a renal tumor. Revue d'Odonto-Stomatologie (Paris) 19(1): 82

Suzuki, H.; Hikiji, W.; Tanifuji, T.; Abe, N.; Fukunaga, T., 2014: Child deaths from injury in the special wards of Tokyo, Japan (2006-2010): a descriptive study. There has been increasing interest in the formal review of child deaths in Japan. In this study we examined the causes and scene information regarding child deaths from injury in Tokyo, the capital of Japan, as preparation for implementation of a...

Vanderschueren Lodeweycky M.; Laurance B.M., 1977: Human chorionic gonadotropin test before surgery for bilateral cryptorchidism. Journal of Pediatrics 91(1): 159-160

Ma, J.J.K.; Tseng, S.S.; Yarascavitch, B.A., 2009: Anterior segment optical coherence tomography for transepithelial phototherapeutic keratectomy in central corneal stromal scarring. To demonstrate the use of anterior segment optical coherence tomography (ASOCT) in planning phototherapeutic keratectomy (PTK) removal of central corneal stromal scarring. A 62-year-old male presented with central corneal scars associated with sig...

Duncan, D.F., 1979: Mental health and health education: an emerging partnership. Urban Health 8(4): 12-13

Zhou HuiZhen; Cao ZiRong; Jiang Xiao, 1999: Dynamic monitoring and pre-warning for basic farmland. Huangtang township, located in the catchment of Tai Lake, Jiangsu Province, China, and also a developed economic area was selected as a test area for this project. A dynamic monitoring and pre-warning system were set up for the project based on GI...