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An actin point-mutation neighboring the 'hydrophobic plug' causes defects in the maintenance of cell polarity and septum organization in the fission yeast Schizosaccharomyces pombe


, : An actin point-mutation neighboring the 'hydrophobic plug' causes defects in the maintenance of cell polarity and septum organization in the fission yeast Schizosaccharomyces pombe. Febs Letters 392(3): 237-241

The fission yeast cps8 mutation gives rise to abnormally enlarged and dispolarized cells, each of which contains several nuclei with aberrant multisepta. Molecular cloning and sequence analysis of the cps8 gene indicated that it encodes an actin with an amino acid substitution of aspartic acid for glycine at residue 273 in the hydrophobic loop that is located between actin subdomains 3 and 4. Fluorescence microscopy using phalloidin and anti-actin antibody revealed changes in the F-actin structure and distribution in the mutant cells. These results indicate that the hydrophobic loop plays an essential role for creating normal F-actin structure, only by which cell polarity and the late mitotic events can be maintained properly.

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PMID: 8774852


Other references

Kosugi, A., 1960: On the food habits of some herons. Miscellaneous Reports of the Yamashina Institute of Ornithology and Zoology, 2(15): 89-98

Schroll, M., 1997: Old age--a trump or a joker?. Ugeskrift for Laeger 159(43): 6339-6339

Muralidhar, R.V.; Panda, T., 1999: Useful products from human placenta. Human placenta is a viable source of many useful products. Some of the products obtained are of use in health care industry. This review article deals with cosmetics, pharmaceuticals, blood products and hormone like substances obtained from human...

Yamamoto M.; Turner T.T., 1989: Proluminal movement of tritiated androgen across the epididymal epithelium in the rat after hypophysectomy and gonadotropin supplementation. The effects of hypophysectomy and gonadotropin replacement on transepithelial movement of 3H-androgen in the rat epididymis were examined by in vivo microperifusion of 3H-testosterone followed by in vivo micropuncture to obtain peritubular and int...

Handy R.D.; Eddy F.B., 1991: Effects of inorganic cations on sodium adsorption to the gill and body surface of rainbow trout oncorhynchus mykiss in dilute solutions. Using the shortest practical exposure time (15 s) to 0.2 mmol.cntdot.L-1 NaCl solutions containing 22Na, over 80% of the Na+ taken up by rainbow trout, Oncorhynchus mykiss, was located on the gill or body surface (adsorption). Infux to the blood (...

Bamborough, P.; Angell, R.M.; Bhamra, I.; Brown, D.; Bull, J.; Christopher, J.A.; Cooper, A.W.J.; Fazal, L.H.; Giordano, I.; Hind, L.; Patel, V.K.; Ranshaw, L.E.; Sims, M.J.; Skone, P.A.; Smith, K.J.; Vickerstaff, E.; Washington, M., 2007: N-4-Pyrimidinyl-1H-indazol-4-amine inhibitors of Lck: indazoles as phenol isosteres with improved pharmacokinetics. 2,4-Dianilino pyrimidines are well-known inhibitors of tyrosine kinases including lymphocyte specific kinase (Lek). Structure-activity relationships at the 4-position are discussed and rationalised. Examples bearing a 2-methyl-5-hydroxyaniline su...

Anonymous, 1977: Treatment of dandruff. Medical Letter on Drugs and Therapeutics 19(15): 63-64

Bhalerao, R.A.; Pinto, A.C.; Bapat, R.D.; Shetty, S.V.; Bhide, P.D.; Waingankar, V.S.; Kirtane, J.M.; Mehendale, V.G.; Shetty, S.D., 1978: Selective transplenic decompression of oesophageal varices by distal splenorenal and splenocaval shunt. The usefulness of selective transplenic decompression of oesophageal varices by distal splenorenal shunt and splenocaval shunt was evaluated in the control of gastrointestinal haemorrhage in patients with portal hypertension of varied aetiology. (...

Egawa, T., 2009: Runx and ThPOK: a balancing act to regulate thymocyte lineage commitment. CD4-positive helper T cells and CD8-positive cytotoxic T cells comprise the majority of T lymphocytes present in secondary lymphoid organs and are essential for acquired immunity. These two populations are derived from common precursors in the thy...

Keppler, D.O.; Schulz-Holstege, C.; Fauler, J.; Reiffen, K.A.; Schneider, F., 1982: Uridylate trapping, induction of UTP deficiency, and stimulation of pyrimidine synthesis de novo by D-galactosone. d-Galactosone (d-lyxo-2-hexosulose) is phosphorylated and metabolized to the uridine diphosphate derivative in AS-30D hepatoma cells and rat liver. These reactions were catalysed in vitro by galactokinase and hexose-1-phosphate uridylyltransferase...