+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

An activator element within the preprotachykinin-A promoter

, : An activator element within the preprotachykinin-A promoter. Molecular and Cellular Neurosciences 5(2): 165-175

The rat Preprotachykinin-A promoter (PPT) directs high levels of expression in dorsal root ganglia (DRG) neurons in culture either endogenously or when linked to a reporter construct. It is not active in any of the established tissue culture cell lines which we have analyzed. To search for transcriptional regulators within this promoter we have started to dissect the promoter into individual elements to determine their function. A DNA element which had previously been suggested to regulate transcription from DNA sequence analysis of the rat PPT promoter occurs at position -200 relative to the major start of transcription within the PPT promoter. The equivalent element from the bovine PPT promoter had previously been proposed to be a cAMP responsive element (CRE). The sequence of this enhancer has similarities with both the AP1 and CRE DNA consensus sequences. We have demonstrated that one copy of this rat PPT element linked to a heterologous basal promoter will enhance transcription in HeLa and PC12 cell lines as well as adult rat DRG neurons grown in culture. It is also demonstrated that the rat PPT element will bind proteins in HeLa nuclear extract distinct from those binding to the well-characterized Gibbon Ape Leukemia Virus (GALV) AP 1 or somatostatin CRE sites by gel retardation analysis. This PPT element, when cloned in a heterologous reporter construct, although showing properties of both AP1 and CRE elements, was functionally distinguished from both the somatostatin CRE element and the GALV AP1 enhancer when these elements were tested in the same reporter construct. This PPT element has a constitutive level of activity in adult rat DRG neurons, which is fivefold higher than that driven by the reporter construct promoter. It is also significantly different from the same reporter construct linked to the somatostatin CRE and analyzed in DRG neurons.


PMID: 8032684

DOI: 10.1006/mcne.1994.1018

Other references

Kim, K-Soon.; Kwon, S-Hi., 2007: Comfort and quality of life of cancer patients. This study aimed to quantify the comfort level and quality of life (QoL) of cancer patients, to identify the variables associated with comfort level and QoL, and to identify the relationship between comfort level and QoL. This was a cross-sectiona...

Kim, K.; Ahn, H.; Park, M.Jeong., 2017: Highly Catalytic Pt Nanoparticles Grown in Two-Dimensional Conducting Polymers at the Air-Water Interface. We report a new approach to the synthesis of uniform, high areal density Pt nanocrystals supported by conducting polymers. The key strategy is the use of ice-templated, two-dimensional polyaniline nanosheets at the air-water interface as a platfor...

Schegolev, G., 1917: Notes spermatologiques. I. Sur la disposition des cellules seminales chez l'Helix pomatia. Revue Zoologique Russe, 1 360-367

Kuehn, D.P., 1991: New therapy for treating hypernasal speech using continuous positive airway pressure (CPAP). The purpose of this report is to introduce a new therapy technique for treating hypernasality. The instrumentation consists of a continuous positive airway pressure (CPAP) device that delivers air pressure by means of a hose and nasal mask assembl...

Nagornyĭ, S.A.; Ermakova, L.A.; Krivorotova, E.Iu., 2013: The specific features of the epidemiology and epizootology of dirofilariasis in Rostov-on-Don and the Rostov region. In the Rostov Region, there have been stable foci of dirofilariasis for a long time (since the 1990s). Two helminth species (Dirofilaria repens, D. immitis) parasitize in definitive hosts. There has been recently a species-specific alteration in t...

Hore, J.K.; Sen, S.K., 1985: Effect of seed treatments on longevity of bael (Aegle marmelos C) seeds. Three days after extraction the seeds were treated in several ways, then dried back to their original weight and stored in alkathene bags at room temperature for up to 132 days. Some seeds were sown 12 days after treatment and others at 132 days....

Chohan, J.; Kaur, J., 1975: Seed mycoflora of sunflower and control of seedborne pathogens. Indian journal of mycology and plant pathology: 5 (2) 210-211

Dong, Q.; Cheng, Z.; Chang, W.; Blackman, B.E.; Conte, F.A.; Hu, J.; Shoback, D.; Miller, W.L., 2010: Naturally-occurring mutation in the calcium-sensing receptor reveals the significance of extracellular domain loop III region for class C G-protein-coupled receptor function. Inactivating mutations of the calcium-sensing receptor (CaSR) cause familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism. Most mutations are clustered in the N-terminal and Cys-rich regions of the extracellular domain (ECD)...

Szekais B., 1992: Treatment approaches for patients with dementing illness. Kiernat, J M (Ed ) Aspen Series in Occupational Therapy: Occupational Therapy And The Older Adult: A Clinical Manual Xv+361p Aspen Publishers, Inc : Gaithersburg, Maryland, Usa Illus 192-219

Vasil'-Eva, V.; Fedotova, S.; Derkach, E.; Tsirlin, Y., 1986: Vacuum rectification of crude furfuryl alcohol. Hydrolysis and wood chemistry USSR: ) 11-14