geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Application of multilayer coil countercurrent chromatography for the study of Vitis vinifera cv. Riesling leaf glycosides


, : Application of multilayer coil countercurrent chromatography for the study of Vitis vinifera cv. Riesling leaf glycosides. Journal of Agricultural and Food Chemistry 41(9): 1452-1457

The glycosidic fraction isolated from Vitis vinifera cv. Riesling leaves by XAD-2 adsorption and methanol elution was separated by multilayer coil countercurrent chromatography (MLCCC) to identify genuine precursors of the off-flavor-causing compound 1,1,6-trimethyl-1,2-dihydronaphthalene (TDN). A two-step procedure utilizing preparative MLCCC in combination with analytical MLCCC yielded two major vine leaf glycosides in pure form, i.e., the beta-D-glucopyranosides of 3-oxo-7,8-dihydro-alpha-ionol (Blumenol C) and 3-oxo-4,5-dihydro-alpha-ionol, respectively. The determination of minor vine leaf constituents in separated MLCCC fractions was achieved after acetylation and subsequent HPLC purification of the per-O-acetylated derivatives. This procedure allowed the identification of 3,4-dihydroxy-7,8-dihydro-beta-ionone 3-O-beta-D-glucopyranoside as a major TDN precursor in Riesling leaves. In addition, the beta-D-glucopyranosides of 3,4-dihydroxy-beta-ionone and zingerone were identified.

US$19.90

DOI: 10.1021/jf00033a019


Other references

Englin, J.B.ker, J., 1995: Briefing series on the 1995 farm bill: conservation reserve and conservation compliance policy. Fact sheet (94-57)

Waldrop, M.M., 1982: Bubbles upon the River of Time. Science 215(4536): 1082-1083

Domoki, J.S.har, J., 1976: Nitrate and nitrite content of vegetables and baby food in Hungary. Elelmiszervizsgalati kozlemenyek: 2 (5 6) 335-345

Robin, J.; Dubayah, R.; Sparrow, E.; Levine, E., 2008: Monitoring start of season in Alaska with GLOBE, AVHRR, and MODIS data. Journal of Geophysical Research 113.G1

Doroshenko, V.G.; Shakulov, R.S.; Kazakova, S.M.; Kivero, A.D.; Yampolskaya, T.A.; Mashko, S.V., 2010: Construction of an L-phenylalanine-producing tyrosine-prototrophic Escherichia coli strain using tyrA ssrA-like tagged alleles. To construct a Phe-producing Tyr(+) Escherichia coli strain, TyrA (chorismate mutase/prephenate dehydrogenase) activity was varied by engineering a proteolytically unstable protein. The tyrA in the E. coli BW25113 was altered to include ssrA-like...

Salem G., 1974: Conformational studies on collagen. Israel Journal Of Medical Sciences: 1465-1466

Tellez, J.; Pichard, R.; Larrain, C., 1951: Treatment of rheumatoid arthritis with nitrogen mustard. Revista Clinica Espanola 42(6): 377-382

Avila, F.W.; Erickson, J.W., 2007: Drosophila JAK/STAT pathway reveals distinct initiation and reinforcement steps in early transcription of Sxl. X-linked signal elements (XSEs) communicate the dose of X chromosomes to the regulatory-switch gene Sex-lethal (Sxl) during Drosophila sex determination. Unequal XSE expression in precellular XX and XY nuclei ensures that only XX embryos will act...

Kawai, H.; Morimoto, S-Ichiro.; Takakuwa, Y.; Ueda, A.; Inada, K-Ichi.; Sarai, M.; Arimura, T.; Mutoh, T.; Kimura, A.; Ozaki, Y., 2016: Hypertrophic Cardiomyopathy Accompanied by Spinocerebellar Atrophy With a Novel Mutation in Troponin I Gene. We report the case of a 66 year-old woman with chronic atrial fibrillation, hypertrophic cardiomyopathy (HCM), and spinocerebellar atrophy (SCA). Her mother and first-born son had died of heart disease at the ages of 65 and 16 years, respectively....

Powell, W.S.; Solomon, S., 1980: Effects of prostaglandins on the adenylate cyclase activity of lungs from fetal rabbits. Prostaglandin E1 (PGE1) and PGI2 stimulate adenylate cyclase activity in lungs from fetal rabbits (29 days of gestation) with EC50 values of 4.4 and 2.6 X 10(-7) M, respectively. PGE2, PGD2, PGF2 alpha, and 6-oxoPGF1 alpha are active only at much...