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Automated analysis of protein NMR assignments using methods from artificial intelligence

, : Automated analysis of protein NMR assignments using methods from artificial intelligence. Journal Of Molecular Biology. 269(4): 592-610

An expert system for determining resonance assignments from NMR spectra of proteins is described. Given the amino acid sequence, a two-dimensional 15N-1H heteronuclear correlation spectrum and seven to eight three-dimensional triple-resonance NMR spectra for seven proteins, AUTOASSIGN obtained an average of 98% of sequence-specific spin-system assignments with an error rate of less than 0.5%. Execution times on a Sparc 10 workstation varied from 16 seconds for smaller proteins with simple spectra to one to nine minutes for medium size proteins exhibiting numerous extra spin systems attributed to conformational isomerization. AUTOASSIGN combines symbolic constraint satisfaction methods with a domain-specific knowledge base to exploit the logical structure of the sequential assignment problem, the specific features of the various NMR experiments, and the expected chemical shift frequencies of different amino acids. The current implementation specializes in the analysis of data derived from the most sensitive of the currently available triple-resonance experiments. Potential extensions of the system for analysis of additional types of protein NMR data are also discussed.


PMID: 9217263

DOI: 10.1006/jmbi.1997.1052

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López-León, T.; Ortega-Vinuesa, J.Luis.; Bastos-González, D., 2012: Ion-specific aggregation of hydrophobic particles. This work shows that colloidal stability and aggregation kinetics of hydrophobic polystyrene (PS) nanospheres are extremely sensitive to the nature of the salt used to coagulate them. Three PS latices and four aggregating electrolytes, which all s...

Mclean, F.C.; Hastings, A.B.ird, 1933: Concentration of calcium ions in biological fluids. Preliminary paper. Brief report of a method of determining Ca ion concentration based on the fact that the frog heart reacts specifically to Ca ions, bound Ca being without effect.

Powell, Ja, 1983: Biology and immature stages of Australian ethmiid moths (Gelechioidea). Journal of research on the Lepidopteraer (pub1983) 20(4): 214-234

Sanseverino, A.M.; Wiedenburg, S.; Fittkau, E.J., 2002: Marauia group: a new species group in the genus Tanytarsus van der Wulp, 1874, from the Neotropics (Diptera, Chironomidae). Adult males and pupae of Tanytarsus marauia sp. nov., T. revolta sp. nov., T. waika sp. nov. and T. xingu sp. nov. are described. The species constitute the T. marauia species group in which the males are characterized by irregularly arranged grou...

Baldwin, C.K.; Lall, U., 1999: Seasonality of streamflow: the upper Mississippi River. The seasonality of the upper Mississippi River (Iowa, USA) streamflow was investigated using a 123 year record of daily flow. This long streamflow series provides an interesting look at the high- and low-flow seasons within the year, their interan...

Grobbee, D.E.; Hofman, A., 1989: Results of intervention studies of blood pressure in childhood and adolescence. Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum 192: 19-31

Mato, S.; Robbe, D.; Puente, N., 2005: Presynaptic Homeostatic Plasticity Rescues Long-Term Depression after Chronic 9-Tetrahydrocannabinol Exposure. Journal of Neuroscience 25(50): 619-27

Mayer, M., 1962: Kultur von Protozoon. Mikrokosmos, 51: 140-142

Sanderson, P.N.; Glen, R.C.; Heide, C.; Hudson, B.D.; Tranter, G.E.; Doyle, P.M.; Harris, C.J., 1993: Conformational studies of a cyclic RGD-containing peptide analogue. Journal of Cellular Biochemistry Supplement 0(17 PART C): 283

Jakowska S., 1975: Cement glands and attachment of octolasmis lowei to the gills of callinectes danae from guanabara bay brazil. Transactions Of The American Microscopical Society: 436