+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Bni1p and Bnr1p: downstream targets of the Rho family small G-proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae

, : Bni1p and Bnr1p: downstream targets of the Rho family small G-proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae. Embo Journal 16(10): 2745-2755

The RHO1 gene encodes a homologue of mammalian RhoA small G-protein in the yeast Saccharomyces cerevisiae. Rho1p is required for bud formation and is localized at a bud tip or a cytokinesis site. We have recently shown that Bni1p is a potential target of Rho1p. Bni1p shares the FH1 and FH2 domains with proteins involved in cytokinesis or establishment of cell polarity. In S. cerevisiae, there is an open reading frame (YIL159W) which encodes another protein having the FH1 and FH2 domains and we have named this gene BNR1 (BNI1 Related). Bnrlp interacts with another Rho family member, Rho4p, but not with Rholp. Disruption of BNI1 or BNR1 does not show any deleterious effect on cell growth, but the bni1 bnr1 mutant shows a severe temperature-sensitive growth phenotype. Cells of the bni1 bnr1 mutant arrested at the restrictive temperature are deficient in bud emergence, exhibit a random distribution of cortical actin patches and often become multinucleate. These phenotypes are similar to those of the mutant of PFY1, which encodes profilin, an actin-binding protein. Moreover, yeast two-hybrid and biochemical studies demonstrate that Bni1p and Bnr1p interact directly with profilin at the FH1 domains. These results indicate that Bni1p and Bnr1p are potential targets of the Rho family members, interact with profilin and regulate the reorganization of actin cytoskeleton.


PMID: 9184220

DOI: 10.1093/emboj/16.10.2745

Other references

Jitendra Singh Rathore, Manoj K.Rai…, 2012: Induction of somatic embryogenesis in gum arabic tree. Factors affecting somatic embryogenesis from immature cotyledon of gum arabic tree [<i>Acacia senegal</i> (L.) Willd.] were investigated. Induction of somatic embryogenesis was influenced by plant growth regulator concentrations and ad...

Waclawski, A.P.; Sinko, P.J., 1996: Oral absorption of anti-acquired immune deficiency syndrome nucleoside analogues. 2. Carrier-mediated intestinal transport of stavudine in rat and rabbit preparations. The intestinal transport and metabolism of stavudine (d4T), a nucleoside analogue of thymidine used in the treatment of AIDS, was studied using single-pass intestinal perfusion (SPIP), intestinal brush-border membrane vesicles (BBMV), and mucosal...

Mcbride, R.P., 1971: Micro-organism interactions in the phyllosphere of Larch. In experiments in vitro, spore germination of Meria laricis was lower in all treatments containing phyllosphere bacteria in soil extracts than in controls with sterile soil extracts, but under the same conditions germination of Cladosporium herbar...

Walzthony D.; Bahler M.; Engel A.; Wallimann T.; Eppenberger H.M., 1985: Myosin head configurations and stem mass determinations. Journal of Muscle Research & Cell Motility 6(1): 82

Koroleva, M.G., 1954: History of pharmacies in Moscow. Aptechnoe Delo 3(6): 37-39

A-Li-Ya; Li-Hong-Tao; Zhou-Ming-Hai; Ha-Si; Tien-Po, 2004: Cloning, expression and purification of human heat shock protein GP96 gene in Escherichia coli. Heat shock protein gp96 is a member of HSP90 families. It can elicits both innate and adaptive immune responses. Generally it is essential to obtain enough amounts of pure, 96 to meet the needs of study and application. But the recombinant gp96 in...

Dailey, J.E., 1958: Ivalon sponge plombage. American Review of Tuberculosis 78(3): 478-484

Munro, Hamish, N., 1977: Tumor host competition for nutrients in the cancer patient. The nutrient needs of malignant cells indicate possible applications to treatment of cancer. The effects of tumors on the host's metabolism involve changes in energy metabolism, including fat and carbohydrate metabolism; disturbances in prote...

Genta, R.M.; Ottesen, E.A.; Poindexter, R.; Gam, A.A.; Neva, F.A.; Tanowitz, H.B.; Wittner, M., 1983: Specific allergic sensitization to Strongyloides antigens in human strongyloidiasis. Reaginic antibodies to antigens from the human parasite Strongyloides stercoralis were studied by histamine release from basophils in 15 patients with chronic uncomplicated strongyloidiasis and in 12 controls (six North Americans with no evidence...

Gales, R., 1988: Sexing adult blue penguins by external measurements. Notornis, 351: 71-75