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Bone marrow-derived elements in the peripheral nervous system. An immunohistochemical and ultrastructural investigation in chimeric rats


, : Bone marrow-derived elements in the peripheral nervous system. An immunohistochemical and ultrastructural investigation in chimeric rats. Laboratory Investigation; A Journal of Technical Methods and Pathology 69(3): 275-282

BACKGROUND: Currently available studies-on the peripheral nervous system (PNS) give little information on the presence, distribution, turnover and function of bone marrow-derived cells such as monocytes, macrophages, and dendritic cells. To address such issues, the present investigation of PNS tissues from bone marrow transplanted chimeras was performed. EXPERIMENTAL DESIGN: Inbred DA rats were lethally irradiated and transplanted with (Lewis times DA)F-1 bone marrow carrying the non-DA major histocompatibility antigens from Lewis rats. The anti-RT-1A-1 antibody (anti-Lewis MHC class 1), 11-69, and the macrophage markers, Ed2 and Ox42 were used to visualize immunohistochemically donor bone marrow-derived cells and resident macrophages of sensory and autonomic ganglia as well as in peripheral nerves. RESULTS: In the ganglia, up to 80% and within peripheral nerves, up to 60% of macrophages were replaced by donor cells within 3 months of established chimerism. Ultrastructurally these cells were located: (a) between the perineurial sheaths, (b) in a perivascular position, or (c) freely dispersed within the endoneurium but outside the basal lamina of Schwann cells and ganglion cells. On the rare occasions wheremyelin and axonal pathology was noted, or during central chromatolysis of ganglion cells, donor marrow-derived cells became positioned under the basal lamina and incorporated tissue debris. CONCLUSIONS: These data suggest a rapid and significant turnover of bone marrow-derived cells within the PNS. Our observations are relevant to the understanding of the pathogenesis of reactive, immunologic, and HIV-associated processes within the PNS.

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PMID: 8377470


Other references

Lowe, R.W.; Hayes, T.D.; Kaye, J.; Bagg, R.J.; Luekens, C.A., 1976: Standing roentgenograms in spondylolisthesis. Lateral roentgenograms of 50 patients with spondylolisthesis were made in the recumbent and standing positions and compared. Thirteen (26%) showed an increase in the percentage of displacement on standing. Those patients with demonstrable change a...

Fedeli E.; Cassertano F.; Maroni G., 1969: Resolution and identification of plasmatic urinary and tissue lipids difficulties methods and proposals. Rivista Italiana delle Sostanze Grasse 46(4): 137-143

Montgomery, C.A.; Latta, G.S.; Adams, D.M., 2006: The cost of achieving old-growth forest structure. Dwindling area of old-growth forest is of concern in many regions of the world. Forest reserves provide one solution. But highly productive timberlands are typically excluded from reserves due to cost. In this study, old-growth forest is defined b...

Logothetopoulos, J.; Valiquette, N.; Cvet, D., 1983: Glucose stimulation of beta-cell DNA replication in the intact rat and in pancreatic islets in suspension culture. Effects of alpha-ketoisocaproic acid, dibutyryl cyclic AMP, and 3-isobutyl-1-methylxanthine in the in vitro system. DNA replication in pancreatic beta-cells was compared in intact rats maintained hyperglycemic by continuous glucose infusion up to 10 days and in rat islets maintained in suspension culture in RPMI 1640 medium up to 12 days. Replicative activity w...

Chen, H.-Lian.; Turkay, M., 2001: Six new species of Leucosiidae from Hainan waters (Crustacea: Decapoda: Brachyura). Acta Zootaxonomica Sinica. Jul; 263: 241-256

Mesko, N.; Bereczkei, T., 2004: Hairstyle as an adaptive means of displaying phenotypic quality. Although facial features that are considered beautiful have been investigated across cultures using the framework of sexual selection theory, the effects of head hair on esthetic evaluations have rarely been examined from an evolutionary perspecti...

Hellstrom, K.; Sjovall, J., 1962: Turnover of deoxycholic acid in the rabbit. A method for studying the turnover of deoxycholic acid in the rabbit is described. The mean values for half-life, pool size, and daily production of deoxycholic acid were 6.8 days, 700 mg, and 73.4 mg, respectively, in 26 rabbits on a diet of conv...

Donovan Peluso, M.; Rosenblum, E.; Cortrese Hassett, A.; Burholt, D.R.; Kornblith, P.L., 1999: Functional urokinase can be measured directly in medium concentrates from cultured tumor cells in vitro. Proceedings of the American Association for Cancer Research Annual Meeting 40: 498, March

Pellerin, D., 1992: Hirschsprung disease. Cirugia Pediatrica 5(2): 57-63

Obst, S.P.; Hall, C.R.; Arnold, M.A., 1996: Economic analysis of Arizona ash sequentially produced in copper-treated or nontreated 021-,25-, and 118-liter containers. Hortscience 31(4): 651