geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

C-fos mRNA pattern and corticotropin-releasing factor neuronal activity throughout the brain of rats injected centrally with a prostaglandin of E2 type


, : C-fos mRNA pattern and corticotropin-releasing factor neuronal activity throughout the brain of rats injected centrally with a prostaglandin of E2 type. Journal Of Neuroimmunology. 70(2): 163-179

The present study investigated the effect of central administration of the prostaglandin of E2 type (PGE2) on the distribution of the immediate early gene (IEG) c-fos mRNA and the transcriptional activity of corticotropin-releasing factor (CRF) and its type 1 receptor in the brain of conscious rats. Adult male rats were sacrificed 30 min and 2 h after a single infusion of PGE2 into the right lateral ventricle (2 micrograms/10 microliters) and their brains cut from the olfactory bulb to the end of the medulla in 30 micrometer coronal sections. mRNAs encoding the IEG c-fos and CRF1 receptor were assayed by in situ hybridization histochemistry using 35S-labeled exonic riboprobes whereas the primary transcript (heteronuclear (hn)RNA) for CRF was detected using intronic probe technology as an index of CRF transcriptional activity. Colocalization of c-fos mRNA within CRF, vasopressin (AVP), and oxytocin (OT) neurons was determined by means of a combination of immunocytochemistry and in situ hybridization techniques on the same brain sections. Thirty min after PGE2 injection, a moderate to strong positive signal for c-fos mRNA was detected in multiple structures of the brain such as the medial preoptic area/organum vasculosum of the lamina terminalis, supraoptic nucleus (SON), parvocellular and magnocellular divisions of the paraventricular nucleus (PVN) of the hypothalamus, central nucleus of the amygdala, nucleus of the solitary tract, dorsal motor nucleus of the vagus, area postrema, dorsal division of the ambiguus nucleus, and throughout the choroid plexus and leptomeninges. A smaller but significant c-fos expression was observed in various structures including the subfornical organ, bed nucleus of the stria terminalis, arcuate nucleus, and periventricular nucleus of the hypothalamus. Two h after treatment with the PG, the signal for c-fos mRNA in most of these brain nuclei vanished. In the parvocellular nucleus of the PVN, c-fos was expressed in CRF-immunoreactive (ir) and OT-ir neurons, whereas in the magnocellular part of that nucleus and in the SON, this transcript was essentially colocalized in OT-ir neurons. Activation of CRF neuroendocrine cells was also associated with an increase in CRF transcription as revealed by the selective presence of CRF primary transcript (hnRNA), which was stimulated only in the PVN but not in any other nuclei in the brains of PGE2-treated rats. Central administration of PGE2 also induced expression of the CRF type 1 receptor in the parvocellular PVN. Taken together, these results provide clear anatomical evidence that central PGE2 injection causes specific and selective expression of c-fos in several brain structures recognized to be activated in the brains of endotoxin-challenged rats. It is therefore possible that PG of E2 type plays a crucial role within the CNS in the interface between the immune and nervous systems to modulate neuroendocrine responses, such as the hypothalamic-pituitary-adrenal axis.

US$19.90

PMID: 8898725

DOI: 10.1016/s0165-5728(96)00114-2


Other references

Allen, R.C., 1938: Temperature and humidity requirements for the storage of dahlia roots. Dahlias were stored under conditions of controlled temp. and humidity. Loss in weight of the root clumps during the storage period was measured at monthly intervals. Most of the loss in weight was due to loss of water. Storage temp. had little eff...

Westermeyer, J.; Nugent, S., 1994: Religiosity and Psychosocial Adjustment Among 100 Homng Refugees. PURPOSE OF THE PAPER. The purpose of this paper is to study the relationship between religiosity and psychosocial adjustment in a group of Asian refugees from Laos. SUMMARY OF METHODS UTILIZED. Subjects consisted of 100 adult Hmong refugees from L...

Rupprechter, A., 1995: Important EU decisions for Austria before the summer break. Important decisions affecting Austria taken by the EU Council in July 1995 included the fixing of the quota for potato starch at 49 100 t, 3000 t more than the Commission's original proposal and the EU wide banning of the antibiotic avoparcin...

Florian, P.Ecaterina.; Rouillé, Y.; Ruta, S.; Nichita, N.; Roseanu, A., 2016: Recent advances in human viruses imaging studies. Microscopy techniques are often exploited by virologists to investigate molecular details of critical steps in viruses' life cycles such as host cell recognition and entry, genome replication, intracellular trafficking, and release of mature...

Swami, K.G.; Ingley, M.N.; Ganvir, B.N.; Bhopale, A.A.; Wankhede, R.B., 2005: Human resource management in papaya nursery. A comparative evaluation is presented of the economics of labour in the cultivation of local and Madhubindu (Honeydew) pawpaw cultivars in nurseries in Maharashtra, India. Data pertaining to the year 1999-2000 were collected from a sample of 49 nu...

Murakami, N.; Mikami, T., 1975: Finding of fusulinids from Nagato tectonic zone. Journal geol Soc Japan, 815: 331-332

Fellmann, N., 1974: The diagnose and the differential diagnose of the monarthritis. Therapeutische Umschau. Revue Therapeutique 31(7): 452-460

Shimada, J., 1934: Experimental scurvy. 19. Urine and blood lactic acid in scorbutic guineapigs. The onset of scurvy in guineapigs did not produce an increase in the urine lactic acid, while the blood level varied within normal limits. An occasional decrease of blood lactic acid was probably due to inanition. Brit. Chem. Abs.

Imshenetskii A.A.; Kozlova T.M.; Lyakh S.P., 1982: The effect of temperature on the ultrastructural organization of the psychrophilic yeast nadsoniella nigra var psychrophilica. EM revealed cytological effects of the lethal action produced by heating (37.degree. C) on the cells of the psychrophilic yeast N. nigra var. psychrophilica. These effects were vacuolization of the cytoplasm, expansion of nuclear pores and disorga...

Hamnström, B.; Gjone, E.; Norum, K.R., 1969: Familial plasma lecithin: cholesterol acyltransferase deficiency. A WOMAN WITH FAMILIAL PLASMA LECITHIN: cholesterol acyltransferase (L.C.A.T.) deficiency showed, like the other reported cases, obvious corneal opacity, proteinuria, and moderate anaemia with a slight haemolytic component. In the plasma the concen...