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CD4 down-modulation during infection of human T cells with human immunodeficiency virus type 1 involves independent activities of vpu, env, and nef


, : CD4 down-modulation during infection of human T cells with human immunodeficiency virus type 1 involves independent activities of vpu, env, and nef. Journal of Virology 70(9): 6044-6053

The human immunodeficiency virus type 1 (HIV-1) genes vpu, env, and nef have all been implicated in modulating the levels of cell surface CD4 on infected cells. To quantitatively assess the relative contribution of each gene product to the regulation of CD4 during HIV infection of Jurkat T cells and peripheral blood mononuclear cells, we have developed an infectious HIV reporter system which expresses different combinations of these genes. To distinguish infected cells in the early or late stages of infection from uninfected cells, these viruses were designed to express human placental alkaline phosphatase with the kinetics of either early or late viral genes. Flow cytometry to detect placental alkaline phosphatase and CD4 in infected cells showed that vpu, env, and nef are independently capable of down-modulation of CD4. As predicted by their respective expression patterns, nef down-modulated CD4 rapidly during the early phase of virus infection whereas vpu and env functioned late in the infection. In both Jurkat cells and peripheral blood mononuclear cells, a combination of the three genes was more efficient than any one or two genes., demonstrating that all three genes are required to achieve maximal CD4 down-modulation. In primary cells, down-modulation of CD4 was less efficient than in Jurkat cells and there was a stronger dependence on nef function for reducing cell surface CD4. HIV therefore has three genes that are able to independently down-modulate CD4; together, they can eliminate the bulk of cell surface CD4.

US$29.90

PMID: 8709227


Other references

Chuprikov, A.P., 1974: Immunological reactivity and functional asymmetry of the human cerebral hemispheres. Vestnik Akademii Meditsinskikh Nauk SSSR: 53-56

Galhotra, A.P.; Gautam, O.P.; Banerjee, D.P., 1977: Trial of some chemotherapeutic agents in bovine anaplasmosis. Therapeutic trials, involving 100 indigenous or cross-bred calves experimentally infected with Anaplasma, were made in India. Reverin (a rolitetracycline compound) given intramuscularly or intravenously for 2 to 5 days [apparently 5 mg/kg body-wei...

Ryall, R.L.; Ryall, R.G.; Howells, A.J., 1976: The ommochrome biosynthetic pathway of Drosophila melanogaster: the Mn2+-dependent soluble phenoxazinone synthase activity. Soluble extracts of Drosophila melanogaster Mg. contain a Mn2+-dependent phenoxazinone synthase activity, which stimulates the conversion of 3-hydroxyanthranilic acid to cinnabarinic acid and of 3-hydroxykynurenine to xanthommatin. The rate of che...

Cutter, V.M., 1951: The cytology of the fungi. Annual Review of Microbiology 5: 17-34

Robinson, J.B., 1964: This I Know: A Rejoinder. Journal of the American Dental Association 68: 613-616

Miraglia, E.; Calvieri, S.; Giustini, S., 2017: Cutaneous lipomatosis: diagnosing a rare condition. Giornale Italiano di Dermatologia E Venereologia 152(6): 678-680

Carvalho, J.P.; Carvalho, F.M.; Pincerato, K.M.; Pereyra, E.A., 2002: Conization, frozen section examination, and planned hysterectomy in the treatment of high-grade cervical intraepithelial neoplasia. We tested the role of frozen section examination of the cone specimen in the evaluation of the resection margin status and to rule out invasion in patients with high-grade cervical intraepithelial neoplasia. Twenty-five patients with cervical intr...

Rimell, F.L.; Kirmani, J.F., 1997: Sinusitis and lower airway reactivity. International Anesthesiology Clinics 35(3): 85-91

Prange, H., 1969: Incidence of claw diseases in. Monatshefte fur Veterinarmedizin: 24 (8) 281-287

Petryniak, M.A.; Potter, G.B.; Rowitch, D.H.; Rubenstein, J.L.R., 2007: Dlx1 and Dlx2 control neuronal versus oligodendroglial cell fate acquisition in the developing forebrain. Progenitors within the ventral telencephalon can generate GABAergic neurons and oligodendrocytes, but regulation of the neuron-glial switch is poorly understood. We investigated the combinatorial expression and function of Dlx1&2, Olig2, and M...